2. Protein A participates in fertilization. Below is a diagram of the protein. It has 468 amino acids. Two mutations have been isolated. Mutation #1 is a single base pair deletion (G in yellow highlights) in the coding region, corresponding to around the 200th amino acid in the protein. Mutation #2 is a 6 base pair deletion (yellow highlights) towards the end of the protein (green highlighted TAA is the stop codon). aal mutation #1 DNA sequence (sense strand): 5'......AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3' corresponding wildtype sequence: 5' ..AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3' aa468 mutation #2 DNA sequence (sense strand): 5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAA actgt... 3' corresponding wildtype sequence: 5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAAactgt... 3' a). (10 points). In the above sequences, the open reading frame is indicated by alternating non-underlined and underlined triplets. Please use the codon table to deduce the amino acid sequence for the region shown in the wildtype protein. Wildtype AA sequence for the region around mutation #1: Wildtype AA sequence for the region around mutation #2: b). (10 points). Please make predictions what molecular change mutation #1 and mutation #2 cause. c). (5 points). Which mutation is more likely to abrogate the protein function? Why?
2. Protein A participates in fertilization. Below is a diagram of the protein. It has 468 amino acids. Two mutations have been isolated. Mutation #1 is a single base pair deletion (G in yellow highlights) in the coding region, corresponding to around the 200th amino acid in the protein. Mutation #2 is a 6 base pair deletion (yellow highlights) towards the end of the protein (green highlighted TAA is the stop codon). aal mutation #1 DNA sequence (sense strand): 5'......AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3' corresponding wildtype sequence: 5' ..AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3' aa468 mutation #2 DNA sequence (sense strand): 5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAA actgt... 3' corresponding wildtype sequence: 5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAAactgt... 3' a). (10 points). In the above sequences, the open reading frame is indicated by alternating non-underlined and underlined triplets. Please use the codon table to deduce the amino acid sequence for the region shown in the wildtype protein. Wildtype AA sequence for the region around mutation #1: Wildtype AA sequence for the region around mutation #2: b). (10 points). Please make predictions what molecular change mutation #1 and mutation #2 cause. c). (5 points). Which mutation is more likely to abrogate the protein function? Why?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...
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Transcribed Image Text:2. Protein A participates in fertilization. Below is a diagram of the protein. It has 468 amino
acids. Two mutations have been isolated. Mutation #1 is a single base pair deletion (G in
yellow highlights) in the coding region, corresponding to around the 200th amino acid in the
protein. Mutation #2 is a 6 base pair deletion (yellow highlights) towards the end of the
protein (green highlighted TAA is the stop codon).
aal
mutation #1 DNA sequence (sense strand):
5'......AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3'
corresponding wildtype sequence:
5'
..AAAATGGTAAAGGCCGTCGTAGGATTCGGCGCT ... 3'
aa468
mutation #2 DNA sequence (sense strand):
5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAA actgt... 3'
corresponding wildtype sequence:
Transcribed Image Text:5'......TACTGCCCATGCCCAGAGAGAAAGCGCAGACGCGTCTAAactgt... 3'
a). (10 points). In the above sequences, the open reading frame is indicated by alternating
non-underlined and underlined triplets. Please use the codon table to deduce the amino
acid sequence for the region shown in the wildtype protein.
Wildtype AA sequence for the region around mutation #1:
Wildtype AA sequence for the region around mutation #2:
b). (10 points). Please make predictions what molecular change mutation #1 and mutation
#2 cause.
c). (5 points). Which mutation is more likely to abrogate the protein function? Why?
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