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- 3Given C6H12O2 chemical formula. chemical shift , intergration, multiplicity and interpretation needed.(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?
- Roughly how far does an infrared beam penetrate into the sample using an ATR-FTIR spectrometer? Select one: 1 pm 1 μm 1 μΜ 1 nm sbiniesnediytem-WA student prepared several solutions of Molecule Bright, each at a different concentration. The absorbance of each solution was measured at 405 nm with a path length of 0.730 cm. The student then plotted the absorbance of each sample vs. its concentration (in mM), and found a best-fit line of y = 3.726x + 0.828. Calculate the molar absorptivity of the Molecule Bright (in M-1 cm-1).8- The fluorescence is mainly resulting from which of the following transitions? (P) n→Ã* (Q) 0→ 0* (R) n→ o* (S) ñ→ ñ* (A) P and S (B) Q and R (C)P only (D) S only IC
- The ultraviolet spectrum of aspirin shows a secondary absorption band at 271 nm. If a solution of aspirin in water, with a concentration of 1×10-4 molar solution is examined at 271 nm, what will be the absorbance reading (ℇ = 1000) and what will be the intensity ratio, IO/I, respectively?Why is it most accurate to measure absorbances in the range A = 0.3 to 2?2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?
- A student creates a sports drink by dissolving 5.0 g of powder in 250 mL of water. The green colour of the drink is due to the presence Green Dye X, which has a lambdamax of 630 nm. A calibration curve (absorbance versus concentration) at lambdamax is created for varying concentrations (mM) of Green Dye X which has the equation y = 26.8x. If 1.0 mL of the sports drink is diluted to 10. mL with water, then the absorbance is measured to be 0.340, what is the mass percent of Green Dye X in the drink powder if the molecular weight is 418.4 g mol-1?Hooke's Law and SpectroscopyWhat effect does increasing the operating frequency of a 1H NMR spectrum have on each value: (a) the chemical shift in δ; (b) the frequency of an absorption in Hz; (c) the magnitude of a coupling constant J in Hz?
