(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: A = In 7 = ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?

Principles of Modern Chemistry
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(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a
cuvette is given by:
10
A = In == ECL
where lo is the incident light intensity, is the transmitted light intensity, e is the
molar absorptivity, c is the concentration of the solution, and L is the path length of
the cuvette.
(1) Light with a wavelength of 800 nm is passed through a cuvette containing a
solution of Coumarin dye. After passing through the cuvette, the light intensity
was reduced to 10% of its initial value.
What is the absorbance of the solution of Coumarin dye?
(ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has
a path length of 1 cm.
What is the molar absorptivity of Coumarin dye at 800 nm, in units of
m² mot¹?
(IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at
800 nm.
How would the absorbance of the solution change, when measured with
400 nm light rather than 800 nm light?
Transcribed Image Text:(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?
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