4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10.¹ to 104, and 100 μl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-³ 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question
4. You have been given 6 new strains of Bacillus species recently isolated from a mining
site. In order to compare the growth patterns of these strains, you set up a 24 hour
growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and
samples were taken after 24 hours. Each sample was then serially diluted from 10-¹ to
104, and 100 µl aliquots from each plated onto nutrient agar plates for enumeration
(using the spread plate method). The plates were incubated overnight and the colony
counts recorded in the table below.
Strain number
B1
B2
B3
B4
B5
B6
10-¹
TMTC
935
TMTC
TMTC
156
570
B1
B2
B3
B4
B5
10-²
B6
TMTC
99
391
TMTC
21
67
Dilution
10-3
786
25
48
670
4
12
10-4
220
Note: TMTC = Too Many To Count
Complete the table below by calculating the number of CFU/ml for each of strains listed above
Strain number
CFU/ml
0
4
112
0
0
Transcribed Image Text:4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10-¹ to 104, and 100 µl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-3 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education