4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10.¹ to 104, and 100 μl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-³ 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0
4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10.¹ to 104, and 100 μl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-³ 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:4. You have been given 6 new strains of Bacillus species recently isolated from a mining
site. In order to compare the growth patterns of these strains, you set up a 24 hour
growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and
samples were taken after 24 hours. Each sample was then serially diluted from 10-¹ to
104, and 100 µl aliquots from each plated onto nutrient agar plates for enumeration
(using the spread plate method). The plates were incubated overnight and the colony
counts recorded in the table below.
Strain number
B1
B2
B3
B4
B5
B6
10-¹
TMTC
935
TMTC
TMTC
156
570
B1
B2
B3
B4
B5
10-²
B6
TMTC
99
391
TMTC
21
67
Dilution
10-3
786
25
48
670
4
12
10-4
220
Note: TMTC = Too Many To Count
Complete the table below by calculating the number of CFU/ml for each of strains listed above
Strain number
CFU/ml
0
4
112
0
0
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