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4. Variants of biological compounds that contain a radioactive element (e.g. 14C, ³H, 32P) in place of a nonradioactive isotope (e.g. ¹2C, ¹H, ³²P) are called "radiolabeled". Radiolabled compounds are utilized by cells and organisms just like non-radiolabeled compounds are, but the presence of the radiolabel allows the metabolic fate of the compound to be tracked. a. Suppose that you want to radioactively label DNA but not RNA in dividing and growing bacterial cells. Which radioactive molecule would you add to the bacterial culture medium? Specify the position of the radioactive atom in the molecule you've chosen. b. Suppose that you want to enzymatically synthesize DNA in which the backbone phosphorus atoms are uniformly labeled with 32P. Which precursors should be added to a solution containing DNA polymerase and template DNA? Specify the position of radioactive atoms in these precursors.

4. Variants of biological compounds that contain a radioactive element (e.g. 14C, ³H, 32P) in place of a nonradioactive isotope (e.g. ¹2C, ¹H, ³²P) are called "radiolabeled". Radiolabled compounds are utilized by cells and organisms just like non-radiolabeled compounds are, but the presence of the radiolabel allows the metabolic fate of the compound to be tracked. a. Suppose that you want to radioactively label DNA but not RNA in dividing and growing bacterial cells. Which radioactive molecule would you add to the bacterial culture medium? Specify the position of the radioactive atom in the molecule you've chosen. b. Suppose that you want to enzymatically synthesize DNA in which the backbone phosphorus atoms are uniformly labeled with 32P. Which precursors should be added to a solution containing DNA polymerase and template DNA? Specify the position of radioactive atoms in these precursors.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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4. Variants of biological compounds that contain a radioactive element (e.g. 14C, ³H, ³2P) in place of a nonradioactive isotope (e.g. ¹2C, ¹H, 32P) are called "radiolabeled". Radiolabled compounds are utilized by cells and organisms just like non-radiolabeled compounds are, but the presence of the radiolabel allows the metabolic fate of the compound to be tracked. a. Suppose that you want to radioactively label DNA but not RNA in dividing and growing bacterial cells. Which radioactive molecule would you add to the bacterial culture medium? Specify the position of the radioactive atom in the molecule you've chosen. b. Suppose that you want to enzymatically synthesize DNA in which the backbone phosphorus atoms are uniformly labeled with 32P. Which precursors should be added to a solution containing DNA polymerase and template DNA? Specify the position of radioactive atoms in these precursors.
Transcribed Image Text:4. Variants of biological compounds that contain a radioactive element (e.g. 14C, ³H, ³2P) in place of a nonradioactive isotope (e.g. ¹2C, ¹H, 32P) are called "radiolabeled". Radiolabled compounds are utilized by cells and organisms just like non-radiolabeled compounds are, but the presence of the radiolabel allows the metabolic fate of the compound to be tracked. a. Suppose that you want to radioactively label DNA but not RNA in dividing and growing bacterial cells. Which radioactive molecule would you add to the bacterial culture medium? Specify the position of the radioactive atom in the molecule you've chosen. b. Suppose that you want to enzymatically synthesize DNA in which the backbone phosphorus atoms are uniformly labeled with 32P. Which precursors should be added to a solution containing DNA polymerase and template DNA? Specify the position of radioactive atoms in these precursors.
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