4. An enzyme-substrate complex has a Kd = 100 nM. A competitive inhibitor with whichof the following Ki values will be most effective at inhibiting this enzyme?A) K₁ = 10 nMB) K₁ = 100 nMC) K₁ = 1000 nMD) None of the above - only the KM is importantBriefly explain:

Answered: 4. An enzyme-substrate complex has a Kg…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

4. An enzyme-substrate complex has a Kd = 100 nM. A competitive inhibitor with which
of the following Ki values will be most effective at inhibiting this enzyme?
A) K₁ = 10 nM
B) K₁ = 100 nM
C) K₁ = 1000 nM
D) None of the above - only the KM is important
Briefly explain:

Expert Solution

Step 1

K_D (Dissociation constant) is the amount of ligand molecules that is required to fill the binding sites of half of the receptors. Thus, a low K_D value indicates that there is high affinity between the ligand and receptor. K_i measures the affinity of inhibitor molecule for the enzyme. It measures the affinity by measuring the inhibitor concentration that is required to produce half of the maximum inhibition. Thus, a low K_i value indicates a high affinity between enzyme and inhibitor.