3. Did your predictions match your results? 4. What may have occurred to cause the difference between your predictions and results? (Hint: Look at your assumptions on pg. 65). 5. Lactase is speeding up what type of chemical reaction-hydrolysis or dehydration? How can the experiment be enhanced? What would you do differently next time? (Note: "noth- ing" is not an acceptable answer.)

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I need help with general biology lab 7 on enzyme denatuation, questions 3-6 ( I will send this question 4 times because the computer will only let me send this with 2 pages, there are 8 pages total)

This is pages 7 and 8

 

These are the last 2 pages and the last page has questions 3-6 on it, which I need help with, thanks

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3.
5.
Did your predictions match your results?
4. What may have occurred to cause the difference between your predictions and results? (Hint:
Look at your assumptions on pg. 65).
6.
Enzyme Denaturation
Lactase is speeding up what type of chemical reaction-hydrolysis or dehydration?
How can the experiment be enhanced? What would you do differently next time? (Note: "noth-
ing" is not an acceptable answer.)
References
Reinking, L.N., J.L. Reinking, and K.G. Miller. 1994. Fermentation, respiration and enzyme specificity:
A simple device and key experiments with yeast. American Biology Teacher, 56: 164-168.
minimum is Y₂
page
for graph
Transcribed Image Text:3. 5. Did your predictions match your results? 4. What may have occurred to cause the difference between your predictions and results? (Hint: Look at your assumptions on pg. 65). 6. Enzyme Denaturation Lactase is speeding up what type of chemical reaction-hydrolysis or dehydration? How can the experiment be enhanced? What would you do differently next time? (Note: "noth- ing" is not an acceptable answer.) References Reinking, L.N., J.L. Reinking, and K.G. Miller. 1994. Fermentation, respiration and enzyme specificity: A simple device and key experiments with yeast. American Biology Teacher, 56: 164-168. minimum is Y₂ page for graph
Expert Solution
Step 1 Difference between prediction and results

Question 3 and 4:

Did your Prediction match your results? What may occurred to cause the difference?

  • Lactaid has lactase enzyme, lactase acts upon lactose producing glucose and galactose. Yeast doesn't have lactase enzyme so it doesn't breakdown lactose it works upon the glucose produced and produces carbondioxide by glycolysis.
  • Lactaid works best around pH 6 to 7. It can work in acidic pH of 2 to 7. Treatment 1,2 and 3 has pH of 2 to 7 so glucose is produced as a result of lactose breakdown and carbon dioxide is produced by yeast in treatment 1,2 and 3. Treatment 4 has pH 12, lactase can't  work at this pH so glucose is not produced as a result carbondioxide will not be released. So the level of carbondioxide remains the same in treatment 4.
  • Predictions are correct in treatment 1,2 and 3. Prediction is wrong in treatment 4.
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