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- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?Lab 2 MicrosCO X Copy of Lab 2 X M Inbox (53,887) x b Answered: 4. 1 x C Search Textbo X + /d/1lq1XGTjeDOK-AZLP5NCDYYPZT3K6_fPLnwx-GvHgLnU/edit# Normal text Arial 12 %00 L 1 2 3. 4. 5. 9. Observing Bacteria in Colonies In fresh or saltwater habitats, cyanobacteria will appear (without a microscope) as very small green lines (as can be seen in this video). Observe the following images of cyanobacteria colonies under 100x and 400x magnification with a light microscope: Anabaena sp. 100x magnification Anabaena sp. 400x magnification As you can see, cyanobacteria grow as two different types of cells: those that are photosynthetic (fixing carbon dioxide to make sugars), and heterocysts (that fix nitrogen to make proteins and nucleic acids). Although these cells are genetically identical, each is able to accomplish this via differences in gene expression, a key concept in biology you will continue exploring this quarter. In the 400x magnification image, you should be able to clearly see two…A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
- The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?The figure below shows the number of bacteria/ml in a series of patient's samples collected and cultured over time, and the resistance of these bacteria to a given antibiotic. This shows all of the following EXCEPT: Bacteria (number/ml) 108 107 106 105 104 103 0 Initiation of antibiotic therapy Bacteria count 1 2 3 5 6 Days Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings. Antibiotic resistance of bacterial population measured by amount of antibiotic needed to control growth 4 7 8 9 10 11 50 40 30 6 Antibiotic resistance (mg/ml) After an initial decline, the number of bacteria eventually rises again as antibiotic- resistant bacteria emerge and multiply At the beginning of antibiotic therapy, most of the bacteria were already resistant to 50 mg/ml of the antibiotic After about 5 days of treatment, the bacteria in the patient's samples were resistant to 50 mg/ml of the antibiotic None of the other four answers (All of these statements are true)Question 1 Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You’re concerned that it’s an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium or a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. What is one experiment you could do, involving culturing the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. What is a procedure you could do, involving making a slide of the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. Without testing anything, how do you know this is not a viral infection?
- TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign outYou perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml
- A sample of raw ground beef initially contained 25 E. Coli bacteria. After six hours, there are 102 400 cells in the sample. What is the growth rate of the E. coli population? Show all work. Express your answer using scientific notation in the form of xyz 10 d #bacteria/hr.Part (a) What metric unit is made when the prefix, da, is combined with the base unit, grams (g)? Write the full word and abbreviation. Part (b) What metric unit is made by combining the prefix, m, with the base unit, meters (m)? Write the full word and abbreviation. Part (c) You are a microbiologist studying a newly found bacterial species, Streptococcus newmexicanus. While studying this species, you examine hundreds of samples and find the average size of this bacterium to be approximately 0.0019 millimeters in length.Convert this measurement into micrometers, with your final answer in scientific notation. Part (d) This bacterium is often found in a chain of bacterial cells connected to each other. The chains can reach lengths of 2.5 mm. Using the information above, calculate how many Streptococcus newmexicanus cells are in a chain 2.5 mm long. Make sure to express your answer in scientific notation & to show your work. Show your work and answer, including units, in the block…= Q Set up Bacteria Growth Experiment 2024 Saved Search Design Layout References Mailings Review View Help Table Design Layout (Body) 11 V A A Aa PEET I Uab x, x AA Font √√ Data Collection Table 1-3: just bacteria sample Day General Observations (color, texture, coverage) 2 No obvious growth. 6 Paragraph V עו # of % Photo of plate colonies Coverage 0% Colors range from white, to light grey, size being roughly ½ cm circles. 1-3 3.65% 2-3 3-3 Large amount of growth since day 4 circles ranging from white grey, black, green, and pink have been observed. White and black areas are about 2 1/2cm around while pink, and green are 1/2cm around. There is also many small white dots covering the majority of the surface area. LX Text Predictions: On Accessibility: Investigate 75° COLLEGE 1-4 40% 2-4 3-3 W FB B Normal No Spacing Heading Heading 2 Title Subtitle Subtle Emphas F7 FB SAMSUNG >/11 D Styles 8 Circles ranging from white grey, black, 1-5 green, and pink have been observed getting bigger.…