Using the data provided, answer the following questions. 3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity?4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance?5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.6. Does it make sense that the relationship between concentration and absorbance should include the origin (0,0) as a point? Explain your reasoning.7. Based on your answer to Question #6, draw the “best-fit” straight line through the data points. How well does this straight line fit the data? Describe the accuracy of the relationship between concentration and absorbance.8. The absorbance of a copper(II) sulfate solution of unknown concentration was measured by colorimetry and found to be 0.250. Use your graph of absorbance versus concentration to estimate the concentration of this unknown solution. **Preparing and Diluting Solutions****Data Table**| Test Tube | Volume of Stock Solution (V₁) | Concentration of Stock Solution (M₁) | Final Volume of Diluted Solution (V₂) | Concentration of Diluted Solution (M₂) | Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) | Absorbance at 635 nm ||-----------|-----------------------------|------------------------------------|-------------------------------------|--------------------------------------|--------------------------------------------------------------------|-----------------------|| 1* | 10 mL | 1.50 M | 10 mL | 1.50 M | 5 | 1.889 || 2 | 3.8 mL | 1.50 M | 10 mL | 0.057 M | 3 | 0.796 || 3 | 2.4 mL | 1.50 M | 10 mL | 0.036 M | 2 | 0.470 || 4 | 5 mL | 1.50 M | 10 mL | 0.075 M | 4 | 0.940 || 5 | 1.53 mL | 1.50 M | 10 mL | 0.02295 M | 1 | 0.326 |\*Test tube #1 contains the initial stock solution.**Explanation:**This table provides information on preparing and diluting solutions across five test tubes. Each test tube's stock solution volume, concentration, final diluted volume, and concentration are recorded along with color comparison rankings from lightest to deepest blue and corresponding absorbance measures at 635 nm.

Note: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since…

3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity? 4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance? 5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.

3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity? 4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance? 5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Using the data provided, answer the following questions.

3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity?

4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance?

5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.

6. Does it make sense that the relationship between concentration and absorbance should include the origin (0,0) as a point? Explain your reasoning.

7. Based on your answer to Question #6, draw the “best-fit” straight line through the data points. How well does this straight line fit the data? Describe the accuracy of the relationship between concentration and absorbance.

8. The absorbance of a copper(II) sulfate solution of unknown concentration was measured by colorimetry and found to be 0.250. Use your graph of absorbance versus concentration to estimate the concentration of this unknown solution.

**Preparing and Diluting Solutions**

**Data Table**

| Test Tube | Volume of Stock Solution (V₁) | Concentration of Stock Solution (M₁) | Final Volume of Diluted Solution (V₂) | Concentration of Diluted Solution (M₂) | Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) | Absorbance at 635 nm |
|-----------|-----------------------------|------------------------------------|-------------------------------------|--------------------------------------|--------------------------------------------------------------------|-----------------------|
| 1* | 10 mL | 1.50 M | 10 mL | 1.50 M | 5 | 1.889 |
| 2 | 3.8 mL | 1.50 M | 10 mL | 0.057 M | 3 | 0.796 |
| 3 | 2.4 mL | 1.50 M | 10 mL | 0.036 M | 2 | 0.470 |
| 4 | 5 mL | 1.50 M | 10 mL | 0.075 M | 4 | 0.940 |
| 5 | 1.53 mL | 1.50 M | 10 mL | 0.02295 M | 1 | 0.326 |

\*Test tube #1 contains the initial stock solution.

**Explanation:**
This table provides information on preparing and diluting solutions across five test tubes. Each test tube's stock solution volume, concentration, final diluted volume, and concentration are recorded along with color comparison rankings from lightest to deepest blue and corresponding absorbance measures at 635 nm.

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