Question 7: read all before answering. A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you’ll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube. tube 1: take some stock, dilute it 1:10 with some water in a new tube = #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube C. First, decide the total volume to make of each tube – keep in mind measurement accuracy (consider preparing 20% more solution than what you will use).
Question 7: read all before answering. A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you’ll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube. tube 1: take some stock, dilute it 1:10 with some water in a new tube = #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube C. First, decide the total volume to make of each tube – keep in mind measurement accuracy (consider preparing 20% more solution than what you will use).
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Question 7: read all before answering.
A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder).
B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you’ll use
5 ml of each dilution for your experiment. Calculate the final concentrations of each tube.
tube 1: take some stock, dilute it 1:10 with some water in a new tube = #1
tube 2: take some of #1, dilute it 1:10 with some water in a new tube
tube 3: take some of #2, dilute it 1:10 with some water in a new tube
tube 4: take some of #3, dilute it 1:10 with some water in a new tube
C. First, decide the total volume to make of each tube – keep in mind measurement accuracy
(consider preparing 20% more solution than what you will use).
D. Then, calculate how much stock, water and final concentration of each.
E. Then, instead of doing a serial dilution, calculate making each of the above solutions using the
weight/volume % solution method.
F. Pros and Cons of serial dilution method vs W/V % method
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