250 150 2 -250 150 100 -100 75 -75 50 -50 37- 37 25 20 15 10 Std Calf Fibrinogen BSA Std Biorad Biorad 161-0374 serum Transferrin 161-0318 -25 -20 -15 -10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data-measured distance moved and calculated Rr values Item Band Distance moved Rr Log 10 Mr (from Calculated Mr (antilog (mm) graph) of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1
250 150 2 -250 150 100 -100 75 -75 50 -50 37- 37 25 20 15 10 Std Calf Fibrinogen BSA Std Biorad Biorad 161-0374 serum Transferrin 161-0318 -25 -20 -15 -10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data-measured distance moved and calculated Rr values Item Band Distance moved Rr Log 10 Mr (from Calculated Mr (antilog (mm) graph) of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![250-
1
2
3
4
5
6
-250
150-
-150
100-
-100
75-
-75
50-
37-
25-
20-
15-
10
10-
Std
Biorad
BSA
Calf
serum
Fibrinogen
Std Biorad
Transferrin
161-0318
161-0374
-50
-37
-25
-20
15
10
2. Measure the distance (in mm) moved for each protein standard band and record
in Table3.6 below.
Table 3.6 Example of table for recording of data - measured distance moved
and calculated Rr values
Item
Band
Distance
moved
(mm)
Rf
Log 10 Mr
(from
graph)
Calculated
Mr (antilog
of the
previous
column)
Sample C
Band 1
Sample F
Band 1
Band 2
Sample B
Band 1
Band 2
Sample T
Band 1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff222a48b-e7b7-4fda-96b5-a5fd8cc06d5c%2F41974cf3-c8a0-4465-9dff-d89ed3b2782e%2Feiuog7k_processed.jpeg&w=3840&q=75)
Transcribed Image Text:250-
1
2
3
4
5
6
-250
150-
-150
100-
-100
75-
-75
50-
37-
25-
20-
15-
10
10-
Std
Biorad
BSA
Calf
serum
Fibrinogen
Std Biorad
Transferrin
161-0318
161-0374
-50
-37
-25
-20
15
10
2. Measure the distance (in mm) moved for each protein standard band and record
in Table3.6 below.
Table 3.6 Example of table for recording of data - measured distance moved
and calculated Rr values
Item
Band
Distance
moved
(mm)
Rf
Log 10 Mr
(from
graph)
Calculated
Mr (antilog
of the
previous
column)
Sample C
Band 1
Sample F
Band 1
Band 2
Sample B
Band 1
Band 2
Sample T
Band 1
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