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2. What are the transmission and absorbance of a blank sample? From other substav 3. Using the data at right: CONCENTRATION a. Calculate the absorbance of each sample. You will need to express the %T values as decimals before calculating the logarithms. (ppm) PERCENT TRANSMISSION ABSORBANCE 0 100.0 100 74.1 (e.g., for T = 25%, A = -log 0.25 = 0.602) 200 54.9 C. b. Use the graph paper on the back of this page (or a spreadsheet) to plot absorbance vs. concentration. Make a proper graph, with labeled axes, uniform scales, etc. Use a ruler to draw the best-fit line. Use the graph to find the concentration of the un- known sample. (Draw dotted lines on the graph showing how you found your answer.) 300 40.7 400 30.1 500 22.3 unknown 60.3

2. What are the transmission and absorbance of a blank sample? From other substav 3. Using the data at right: CONCENTRATION a. Calculate the absorbance of each sample. You will need to express the %T values as decimals before calculating the logarithms. (ppm) PERCENT TRANSMISSION ABSORBANCE 0 100.0 100 74.1 (e.g., for T = 25%, A = -log 0.25 = 0.602) 200 54.9 C. b. Use the graph paper on the back of this page (or a spreadsheet) to plot absorbance vs. concentration. Make a proper graph, with labeled axes, uniform scales, etc. Use a ruler to draw the best-fit line. Use the graph to find the concentration of the un- known sample. (Draw dotted lines on the graph showing how you found your answer.) 300 40.7 400 30.1 500 22.3 unknown 60.3

Principles of Instrumental Analysis
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter20: Molecular Mass Spectrometry
Section: Chapter Questions
Problem 20.14QAP
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2. What are the transmission and absorbance of a blank sample? From other substav 3. Using the data at right: CONCENTRATION a. Calculate the absorbance of each sample. You will need to express the %T values as decimals before calculating the logarithms. (ppm) PERCENT TRANSMISSION ABSORBANCE 0 100.0 100 74.1 (e.g., for T = 25%, A = -log 0.25 = 0.602) 200 54.9 C. b. Use the graph paper on the back of this page (or a spreadsheet) to plot absorbance vs. concentration. Make a proper graph, with labeled axes, uniform scales, etc. Use a ruler to draw the best-fit line. Use the graph to find the concentration of the un- known sample. (Draw dotted lines on the graph showing how you found your answer.) 300 40.7 400 30.1 500 22.3 unknown 60.3
Transcribed Image Text:2. What are the transmission and absorbance of a blank sample? From other substav 3. Using the data at right: CONCENTRATION a. Calculate the absorbance of each sample. You will need to express the %T values as decimals before calculating the logarithms. (ppm) PERCENT TRANSMISSION ABSORBANCE 0 100.0 100 74.1 (e.g., for T = 25%, A = -log 0.25 = 0.602) 200 54.9 C. b. Use the graph paper on the back of this page (or a spreadsheet) to plot absorbance vs. concentration. Make a proper graph, with labeled axes, uniform scales, etc. Use a ruler to draw the best-fit line. Use the graph to find the concentration of the un- known sample. (Draw dotted lines on the graph showing how you found your answer.) 300 40.7 400 30.1 500 22.3 unknown 60.3
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