NAME: LAB SECTION: EXPERIMENT 21: Spectroscopy of Dyes POSTLAB EXERCISE 1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy- gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra- tion of hemoglobin in the solution? 2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm? nohntooote 196

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NAME:
LAB SECTION:
EXPERIMENT 21: Spectroscopy of Dyes
POSTLAB EXERCISE
1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy-
gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used
had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra-
tion of hemoglobin in the solution?
2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm?
nohntooote
196
Transcribed Image Text:NAME: LAB SECTION: EXPERIMENT 21: Spectroscopy of Dyes POSTLAB EXERCISE 1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy- gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra- tion of hemoglobin in the solution? 2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm? nohntooote 196
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