(2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, à (x-axis), to determine the Amax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 500% 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 300 400 700 800

(2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, à (x-axis), to determine the Amax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 500% 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 300 400 700 800

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The absorbance of a mixture containing compounds A and B was to have an absorbance of 0.24 at 250 nm and 0.965 at 375 nm using a cell with a pathlength of 1 cm. The molar absorptivity of A and B at the wavelengths are given in the table (M-1cm-1 or mol-1 L cm-1). Sample & @250 nm & @375 nm 850 2000 500 2400 Determine the concentration of each compound. A B
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Absorbance measurements in the range of A = 0.3 - 2 are considered the most accurate. Why are absorbance measurements of 0.05 and 2.5 considered inaccurate? If the absorbance is too high (A = 2.5), the signal difference between the sample and the reference is too small for an accurate measurement. At A = 2.5, the transmission is too high for an accurate measurement. The difference in the absorbance of the sample and reference is too small at A = 0.05. At A = 2.5, the detector is saturated because too much light is reaching the detector. At A = 2.5, a sufficient amount of light does not reach the detector for an accurate measurement.
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Define the following terms: spectrophotometer, absorbance, pathlength, & (also known as molar absorptivity), concentration, transmittance, Beer's law. RODUCTION: