14 12 10 8. 6 4 1 2 3 Moles -OH added per mole histidine 2. Hd

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Histidine is an amino acid with three titratable groups: an -NH3+ group (pKa = 9.2), a -COOH group (pKa = 1.8), and an imidazole (amine-like) group (pKa = 6.0). The titration curve for histidine is shown below with four points highlighted. (a) Identify which point on the titration curve corresponds to the pKa for each of the titratable groups, and which point corresponds to the pI. Explain your choices. (b) Calculate the value of pI for histidine

14
12
10
8.
6
4
1
2
3
Moles -OH added per mole histidine
2.
Hd
Transcribed Image Text:14 12 10 8. 6 4 1 2 3 Moles -OH added per mole histidine 2. Hd
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