12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, no organisms are seen. What did the student do wrong when making the smear?
Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: What is the purpose of fixing a smear?
A: Smear- a smear is a thin layer of a sample such as bacterial cells or blood spread evenly on a glass…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…
A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…
Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…
A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…
Q: 3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under…
A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…
Q: 13. A student makes a smear by applying a LARGE amount of bacteria onto the slide. A simple stain is…
A: INTRODUCTION You should be very careful while making the smears. It should be very thin, so that you…
Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…
A: Introduction: Acid-fast staining is a type of differential staining technique that was first…
Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…
A: Introduction Capsule is the outer most covering, which is present even outside the cell wall. This…
Q: Which of the following are true about a differential stain. There may be more than one answer!…
A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…
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Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: In your own words, explain how gram staining is done and how it works.
A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…
Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…
A: Option b
Q: 14. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but…
A: Simple stains is to add one stain to a fixed smear slide, let it sit, rinse it off, let it dry, and…
Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…
A: The microorganisms that can be found in several areas of the habitat and come under the category of…
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: 7. Give an example of a differential stain. 8. What is a medium? 9. What is the definition of a…
A: Staining technique is a method in which microscopic sample including bacteria or any other cells are…
Q: What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide…
A: The staining of microorganisms is very much important that helps us in identifying the morphology…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: You have a microscope with a resolution of 0.5 um. Your bacteria on your sample are 0.3 um apart on…
A: The light microscope is a versatile instrument used to study a wide variety of types of specimens…
Q: This part of the staining process helps the primary stain to remain in the cell during…
A: Staining of biological samples is a very useful process for detailed observation and measurement of…
Q: After performing the gram stain you observe your result as gram negative. To help confirm this…
A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…
Q: The first step in identifying an unknown bacterium is to __________. culture your unknown on…
A: Bacteria are the minute microorganisms that are unicellular and have a cellular level of…
Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…
A: After a clinical sample is received in the laboratory, the first step often is to do a mandatory…
Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…
A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…
Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…
A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.
Q: What is the advantage of gram staining over simple staining
A: The biochemical techniques that use different stains or dyes to color the specimens are known as…
Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…
A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…
Q: The GRAM stain method was applied on both pictures. What is the result found on each of them? A A.
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram…
Q: Differential Staining B: Negative staining ~ thin smear C: Endospore Stain
A: Endospore staining: Endospore staining is a method used to selectively stain bacterial endospores.…
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- You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleIn your own words, explain how gram staining is done and how it works.
- a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantWhat is the purpose of fixing a smear? Mark all that apply: To kill the bacteria so they aren't harmed by the staining method O To kill the bacteria to make the slide safer to handle O To attach the bacteria to the slide To break down the cell wall in order to make the cells accept stain To cause the cells to shrink and become distorted Microsoft Bing 11:30 AM do 87 F Sunny 9/14/2021
- If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodineThe first step in identifying an unknown bacterium is to __________. The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your cultureWhat is the identification of the unknown bacteria
- After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative Stain