**Question 11**Chambers and coworkers have reported [NAD⁺] and [NADH] concentrations in yeast mitochondria as 20 mM and 0.3 mM, respectively. Consider the Malate Dehydrogenase reaction below:\[ \text{Malate} + \text{NAD}^+ \rightarrow \text{Oxaloacetate} + \text{NADH} + \text{H}^+ \quad \Delta G'^{0} = +29.7 \, \text{kJ/mol} \]If the Malate concentration in yeast mitochondria is 0.4 mM, what is the maximum concentration of oxaloacetate needed to make the reaction exergonic at pH 7.0 and 37°C?- A. \(7.14 \times 10^{-5}\)- B. \(2.04 \times 10^{-4}\)- C. \(8.17 \times 10^{-6}\)- D. \(5.45 \times 10^{-8}\)- E. \(2.63 \times 10^{-7}\)- F. \(4.69 \times 10^{-8}\)- G. \(4.90 \times 10^{-7}\)- H. \(3.13 \times 10^{-6}\)- I. \(5.05 \times 10^{-6}\)

As the cell's primary energy-producing metabolic pathway, the tricarboxylic acid cycle provides most…

Answered: 11. Chambers and coworkers have…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Assume that 2.5 ATPs are generated per NADH and 1.5 ATPs per FADH2. How many ATPs are generated from the FADH2 and NADH molecules from each repetition of the ββbeta-oxidation pathway? Express your answer as an integer.
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Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data:
Some cofactors that participate in the reactions of the citric acid cycle are indicated by letters. Identify the position of positions of NADH in the cycle by selecting the appropriate letter or letters designating its position(s) in the cycle diagram. COA CH, HO--Co, CH čo, 8 CO но-сн CH HC -Co, CO HO-CH LA Citric acid cycle H,0 co, -B COA | 4 Co, čo D.
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