10)Examine each of the errors below, discuss how the error would affect the separation. Describe how each error would impact the efficacy of the purification (better separation, worse separation, no effect). (Error) a) Overbunched cotton ball at bottom of column b) The silica gel is not level when the sample is loaded onto the column c) Half as much silica gel was used as was indicated in the procedure d) Smaller fractions are collected (less volume in each test tube with test tubes switched more frequently)

10)Examine each of the errors below, discuss how the error would affect the separation. Describe how each error would impact the efficacy of the purification (better separation, worse separation, no effect). (Error) a) Overbunched cotton ball at bottom of column b) The silica gel is not level when the sample is loaded onto the column c) Half as much silica gel was used as was indicated in the procedure d) Smaller fractions are collected (less volume in each test tube with test tubes switched more frequently)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: When should you load your concentrated pigment solution onto your column in column chromatography?…

A: The correct time to add the concentrated pigment solution in the column is ' when the eluent is just…

Q: When a mixture of A and B were subjected to paper chromatography, A moved up higher than B. However,…

A: The question is based on the concept of chromatography. It is a method of separation and…

Q: Explain

A: In TLC analysis we put solvent below the sample spot .

Q: This experiment is about column chromatography of fluorenone. Complete the table

A: The question is based on the concept of IR spectroscopy. We have to identify characteristic peaks…

Q: Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the…

A: A thin layer chromatography (TLC) is used to seperate components of mixture using thin stationery…

Q: i) What two criteria should you ensure about your melting point sample before you fill the melting…

A: Since you have posted multiple questions, but we will solve first question for you. For remaining…

Q: Please don't provide handwritten solution ....

A: Thin layer chromatography (TLC) is a method of separating components of the mixture. It is based on…

Q: 1. Substances that interact more strongly with the mobile phase will tend to elute out more slowly.…

A: The mobile phase can be either liquid or gas in chromatography. It is passed through a…

Q: When a mixture of A and B were subjected to paper chromatography, A moved up higher than B.…

A: Paper chromatography technique is used to separate the compound that present in the mixture.

Q: a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids…

Q: What is meant by retention time in gas chromatography. In what order will they come hexane, ethyl…

A: The process of separation of various constituent of a mixture in zone or in phases is known as…

Q: Which substance is best to run the column chromatography? a)The solvent that is very polar B)The…

Q: 1. For the following chromatography state what the mobile phase is, stationary phase is, and what…

A: Chromatography is a versatile analytical technique used for the separation and identification of…

Q: Discuss which conditions are most effective for separating the four compounds, making reference to…

A: To effectively separate the four compounds, different chromatographic techniques can be used based…

Q: Thin layer chromatography experiment. Show calculations for the Rf of each spot you observed.

A: see the explanation partExplanation:

Q: 1. Is external force present to influence the analysis? Yes or No? 2. If the solvent is chloroform,…

A: TLC is a good tool for the separation of the sample mixtures. In this technique, the sample will…

Question

10)Examine each of the errors below, discuss how the error would affect the
separation. Describe how each error would impact the efficacy of the purification
(better separation, worse separation, no effect). (Error)
a) Overbunched cotton ball at bottom of column
b) The silica gel is not level when the sample is loaded onto the column
c) Half as much silica gel was used as was indicated in the procedure
d) Smaller fractions are collected (less volume in each test tube with test tubes
switched more frequently)

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

DATA REPORT FORM Procedure A: Ink Analysis by Paper Chromatography 1. Attach the chromatography paper results here. Name 2. Calculate the Rf value for each ink/marker sample. If there are more than one spot in the sample, calculate and list Rf for all components. Procedure B: Identification of Unknown Analgesic Drugs 1. Sketch the best results of the TLC plate here, including unknown sample and known compounds. 11
You are planning on performing column chromatography. You ran a TLC plate (below) on your crude mixture to test out the solvent system and stationary phase you plan to use for your column. What order would you expect the components to come off the column? Options: Red (C) first, then black (B) . Blue (A) won't come off the column Not enough information to determine Red first (C) , then black (B) , then blue (A) Blue (A) first, then black (B) . Red (C) won't come off the column Blue (A) first, then black (B) , then red (C)
Chemistry Question 10 4 According to the troubleshooting section for column chromatography in the laboratory textbook, select all that applies to why separation would not occur. Incorrect selections deduct points! The adsorbent surface at the top of the column is not flat and horizontal. Sample may have been overloaded onto a small column. Polarity of starting solvent for elution is too polar. Too much solvent was used for elution
QUESTION 15 To improve the accuracy of our linear fit between absorbance and concentration, a. add an extra data point to your linear regression where you make the assumption that pure water has an absorbance of zero. b. make sure that you choose a "Linear Regression" curve fit. c. ensure that there are no air bubbles in the cuvette. O d. use the data points only at the lower end of the concentration range because Beer's Law is not applicable for higher concentrations. QUESTION 16 What laboratory equipment do you need to prepare a 0.250 M stock solution of NaCI? O a. buret, graduated cylinder, analytical balance b. beaker, volumetric flask O. volumetric pipet, analytical balance d. analytical balance, volumetric flask
Please Explain clearly Why couldn’t you use acid-base extraction to separate a ketone and an alcohol? why do we use column chromatography instead? Please explain clearly the benefits of both methods and why is one method preferred over the other.
None
5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.
what is the effect on the chromatographic peak if a. inserting/adding sample too slow b. column temperature is too high
See image below