What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?
What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?
Chapter89: Thin-layer Chromatography
Section: Chapter Questions
Problem 1P
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