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What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?

What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?
Transcribed Image Text:What difficulty may result if a. chromatographic column is not placed in a vertical position? b. the liquid level of the eluent is allowed to drop below the top of the column? Petroleum ether followed by dichloromethane is used to separate fluorene and 9-fluorenone. Why would reversing the order in which these solvents are used be unwise? What is the best experimental procedure to use in choosing an eluting solvent for column chromatography?
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