1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?
1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?
![e
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Insert Draw Design Layout
X
• Α' Α'
Calibri (Bo...
B I U ab X₂ X²
CONTROL
CFTR-
14
CRISPR
f1 202 words IX
V (mv)
You are employed in a gene therapy laboratory to test the effectiveness of a vector for
correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to
correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was
performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation
which were transformed with either a control vector consisting of an inert CRISPR-CAS-9
construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR).
The following transepithelial voltage values were obtained for the drug regimen shown
under open circuit and current clamp (injected current of 10μA) conditions:
AVRE (mv)
Baseline
Amiloride
-78
-2
8
16
800
1600
5.7777
98
1
-38
-6
9
27
900
2700
42
2
R(S2.cm-2)
I(μA.cm²)
Vte (mv)
AVRE (MV)
References » Tell me
Aa | A
AA.
R(S.cm-2)
Document13
A
Paragraph Styles
tv
Focus
E
2
Forskolin
-4
-23
Comments
6
600
D
38
Dictate Sensitivity
=
Inh172
(uA.cm2)
Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR
channels/μm² in the "CONTROL" and 81 CFTR channels/μm² in the "CFTR-CRISPR".
Calculate the number of moles of Cl- transported per channel per second for both
treatments. Note that there are 1x108 μm² in each cm²
-3
13
1300
2
-9
11
1100
Editing 15
8
C
E
Edit](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F129c8cd9-b844-4154-8ab6-95cb4ac54648%2F89b80c6a-6efc-4208-bbcb-68b977b992a7%2Fd8int4_processed.jpeg&w=3840&q=75)
Transcribed Image Text:e
AutoSave OFF
Insert Draw Design Layout
X
• Α' Α'
Calibri (Bo...
B I U ab X₂ X²
CONTROL
CFTR-
14
CRISPR
f1 202 words IX
V (mv)
You are employed in a gene therapy laboratory to test the effectiveness of a vector for
correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to
correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was
performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation
which were transformed with either a control vector consisting of an inert CRISPR-CAS-9
construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR).
The following transepithelial voltage values were obtained for the drug regimen shown
under open circuit and current clamp (injected current of 10μA) conditions:
AVRE (mv)
Baseline
Amiloride
-78
-2
8
16
800
1600
5.7777
98
1
-38
-6
9
27
900
2700
42
2
R(S2.cm-2)
I(μA.cm²)
Vte (mv)
AVRE (MV)
References » Tell me
Aa | A
AA.
R(S.cm-2)
Document13
A
Paragraph Styles
tv
Focus
E
2
Forskolin
-4
-23
Comments
6
600
D
38
Dictate Sensitivity
=
Inh172
(uA.cm2)
Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR
channels/μm² in the "CONTROL" and 81 CFTR channels/μm² in the "CFTR-CRISPR".
Calculate the number of moles of Cl- transported per channel per second for both
treatments. Note that there are 1x108 μm² in each cm²
-3
13
1300
2
-9
11
1100
Editing 15
8
C
E
Edit
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