1. provide a solution using a table, listing all the specific individual reactions that will generate ATP either by substrate level phosphorylation (SLP) or by oxidative phosphorylation (OP). 2. Shall the given question require a shuttle system, please use both and indicate the net ATP for each. 3. Get the net ATP for each question given you. The second picture is for your reference in format.
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- Calculate the total ATP produced in the catabolism of glucose. Follow the table provided below. If NADH = 3 ATP, If NADH = 2.5 АТР, FADH, 1.5 FADH, = 2 ATP ATP Glycolysis Pyruvate shuttle Krebs cycle ETC TOTAL If NADH = 3 ATP, If NADH = 2.5 %3D FADH2 = 2 ATP ATP, FADH2 = 1.5 ATP Alcohol Fermentation Lactic acid fermentationU Select all of the following half reactions that require energy (work) to proceed as written. NAD+ + 2HNADH + H+ glucose + inorganic phosphate glucose-6-phosphate + H₂O › DETAILS O=d-0 OH NADH+H NAD+ + 2H OH o-p-o-C-CH3 + H2O -0-8-CH3 PREVIOUS ANSWERS ATP+ H₂O ADP + P₁ H قمر H (not balanced) (not balanced) O 0-4-OH + HO-CH₂ 0 xThe Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)
- Which reaction below in the glycolytic pathway has a highly unfavorable delta Gº' but a favorable delta G under physiological conditions? 1,3-Bisphosphoglycerate + ADP3-Phosphoglycerate + ATP O2-phosphoglyerate → phosphoenolpyruvate Ofructose-1,6-bisphosphate dihydroxyacetone phosphate + glyceraldehyde-3-phosphate Ofructose-6-phosphate → fructose-1,6-bisphosphateCalculate the biochemical standard cell potential for the oxidation of NADH by molecular oxygen O2 + 2NADH + 2 H+ → 2H2O + 2NAD+The enzyme lactate dehydrogenase catalyzes the reversible reaction lactate (CH3 CHOHCOO )+NAD* рyruvate (CH3 COCOO) + NADH + H+ Given the standard reduction potentials CH3 СОСОО + 2H+ + 2е CH3 CHОНСО" E°'= -0. 185 V NADŤ + H+ + 2e- → NADH = -0. 320 V E°' would you expect the reaction to be spontaneous in the forward or reverse direction under biochemical standard state conditions? How could you change the reaction conditions to force the reaction to be spontaneous in the opposite direction? Please explain your answer.
- The glyceraldehyde-3-phosphate dehydrogenase reaction of glycolysis, (glyceraldehyde-3- phosphate → 1,3 bisphosphoglycerate) (Choose all that apply) O conserves energy by transfer of a proton H* to NAD+ forming NADH O has a high energy thioester intermediate on the enzyme/substrate complex O mechanistically uses covalent catalysis O conserves energy in the formation of a oxygen phosphoric ester O conserves energy in the formation of a carboxylic-phosphoric mixed anhydride O mechanistically requires the coenzyme thiamine pyrophosphate (TPP) to act as an electron sinkMake a concept map using all of the following terms: GlycolysisOxidation of PyruvateCitric Acid CycleElectron Transport ChainChemiosmosisGlucoseOxidative Phos.Substrate level phosphatePyruvateacetyl-CoACO2OxygenWaterreduced elec. carriers (NADH)oxidized elec. carriers (NAD+)High enerGy Elec.Low enerGy Elec.H+ GradientADP + PiATPBegining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potential
- dtermoine numberof ATPS GENERATED FROM COMPLETE oxidation of fructose-6-phosphate isocitrate stearidonic acid [18 carbons triangle 6,9,12,15] indicate where everything comes from ex ATPS FROM GLYCOLYSIS , NADH FROM TCA ETC LIST ALLSonicating a suspension of mitochondria produces submitochondrial particles derived from the innermitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of themitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electrontransfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidativephosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH ofthe fluid in which the submitochondrial particles are suspended? Why?Complete and identify the enzymes involved in the following pathways (13 pts): P O dihydroxyacetone-P DHAP {^{+E^EX). f ATP ADP ATP ADP glucose glucose-6-P G-6-P fructose-6-P F-6-P fructose-1,6-bisP F1,6-BP NAD+ NADH glyceraldehyde-3-p* GAP 56 CZ-OH С-он -OH ç=O ÷E ATP CH3 ADP pyruvate P-enolpyruvate 2-P-glycerate 3-P-glycerate PEP 2PG 3-PG 1,3-bisP-glycerate 1,3 BPG A. Glycolytic Pathway (Embden-Meyerhof Pathway) 10 ATP ADP CH₂