1. Protein that will most strongly bind to an anion exchange column: [ Select] 2. Protein that will elute last in gel filtration chromatography: [ Select ] 3. Protein that will elute first in hydrophobic interaction chromatography: [ Select ] 4. Protein that will elute last in a carbohydrate containing column: [ Select )

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
100%

Just choose from Proteins A-D. Thank you!

1. Protein that will most strongly bind to an anion exchange column:
[ Select ]
2. Protein that will elute last in gel filtration chromatography:
[ Select ]
3. Protein that will elute first in hydrophobic interaction chromatography:
[ Select ]
4. Protein that will elute last in a carbohydrate containing column:
[ Select ]
Transcribed Image Text:1. Protein that will most strongly bind to an anion exchange column: [ Select ] 2. Protein that will elute last in gel filtration chromatography: [ Select ] 3. Protein that will elute first in hydrophobic interaction chromatography: [ Select ] 4. Protein that will elute last in a carbohydrate containing column: [ Select ]
Consider a mixture comprised of the following proteins below:
-----
Charge Affinity to a!
!MW
Protein;
(pH
(kDa)
7.4)
specific %Polarity
ligand
A
| 760
-3
None
45
B
| 300
+1
Carbohydrate
70
80
-1
None
60
dream
D 55
+2
None
25
C.
Transcribed Image Text:Consider a mixture comprised of the following proteins below: ----- Charge Affinity to a! !MW Protein; (pH (kDa) 7.4) specific %Polarity ligand A | 760 -3 None 45 B | 300 +1 Carbohydrate 70 80 -1 None 60 dream D 55 +2 None 25 C.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON