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1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.

1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.
Transcribed Image Text:1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.
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