1 View and DRAW observations of prepared slides: Bacteria Special Stains a. Endospore Stain: Clostridium tetani and Clostridium botulinum-Label: endospores, note location of endospore for each species b.) Flagella Stain: Proteus vulgaris and Spirillum-MY- volutans-Label: flagella, identify flagella arrangement on each type of organism. P
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- I U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III1 Figure 3. Two different types of bacterial colonies on an agar plate. Using microbiology terms, describe fully the colonial morphology of the two colonies shown above. A full description will include texture, transparency, color, and form (size, overall shape, margin, and elevation). Colony 1 Colony 2 2.Culture Code COLONY MORPHOLOGY GRAM STAIN Name the Bacterium Color Form Surface Opacity Cell color Cell shape Cell Grouping G+ or G- A B C D E
- Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of MethodWhich of the following is true about cell wall of gram-positive bacteria? O Not loss primary stain after alcohol O It consists of multiple peptidoglycan layer O Option 5 O It is thicker than that associated with gram-negative bacteria. All of these45years old male had been working e his garden when he scratched the knuckle of his right aram during digging in ground with a spade,he immediately washed the area but noticed that the knuckle became red,warm to the touch and swollen.he went to hospital,the doctor ordered a hram stain and culture on the pus extracted from the wound. As a microbiologist describe what you can see in on the gram stain taken from the wound site.give a priliminary report on what you can see on culture plates.what is prbable genus and species? Name 2 tests that can be done to positively identify this organism. What are the media used to culture the sample ,and what type of hemolysis seen?
- Negative Stain Name the bacterium used in negative stain Describe morphology Name the disease caused by this bacterium Describe what you observe under microscope in negative staining.Picture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????Clostridium Botulinum Physical caracteristics Shape Arrangement Gram status? Acid fast ? Capsule? Produce Endospore? Environmental Condition required for the growth Oxygen Which of these is: Obligate aerobe, obligate anaerobe, facultative anaerobe. Temperature Which of these is: Psychrophile, Mesophile, Thermophile pH Which of these is: Acidophile, Neutralophile, Alkaliphile
- Search for the following bacteria and complete the table below. Bacterium Stain Description or sketch of cells with the specified feature Flavobacterium capsulatum Capsule stain Corynebacterium diphtheriae Methylene blue (metachromatic granules) Mycobacterium tuberculosis Acid fast stainNegative staining is useful for OA.motility purposes OB.visualizing morphology OC.flagella detection OD.identifying the capsuleIn the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple