**Pre-Lab Questions - DNA Extraction and Quantification**1) **Use the DNA origami model to determine the molecular formula for a base pair unit consisting of adenine, thymine, two deoxyribose, and two phosphates.** Molecular formula: _______________2) **Determine the molecular formula for a base pair unit consisting of cytosine, guanine, two deoxyribose, and two phosphates.** Molecular formula: _______________3) **What is the molecular weight of the base pair unit in question 1?** Molecular weight: _______________4) **What is the molecular weight of the base pair unit in question 2?** Molecular weight: _______________5) **What is the average weight of a base pair unit, assuming the base pair units in questions 3 and 4 occur with equal frequency?** Average weight: _______________6) **What percentage of the average weight of a base pair unit is due to phosphorus?** Percentage: _______________(Note: This document does not contain any graphs or diagrams.) The image depicts the molecular structure of a segment of DNA, focusing on the arrangement of nucleotide pairs and their hydrogen bonding. ### Diagram ExplanationThe diagram shows the complementary base pairing within DNA's double helix structure. There are two strands, each oriented in opposite directions, indicated by the 5’ to 3’ and 3’ to 5’ designations. Each nucleotide is comprised of a sugar-phosphate backbone connected to a nitrogenous base.#### Key Features:1. **Nucleotide Bases**: The nitrogenous bases thymine (T), adenine (A), cytosine (C), and guanine (G) are highlighted. - **Adenine (A)** pairs with **thymine (T)**. - **Cytosine (C)** pairs with **guanine (G)**. - These pairings are held together by hydrogen bonds.2. **Backbone Structure**: The phosphate-sugar backbone is illustrated as connected circles and lines running along each DNA strand.3. **Complementary Pairing**: - Thymine and adenine are linked by two hydrogen bonds. - Cytosine and guanine are linked by three hydrogen bonds. - These bonds are indicated by dashed lines between base pairs.4. **DNA Grooves**: - The "minor groove" and "major groove" of the DNA double helix are labeled at the bottom, indicating the spaces between the twisting strands where proteins often interact with the DNA.This depiction is informative for understanding DNA’s function in genetic coding and replication, as the sequence of these bases encodes genetic information.

A DNA base pair unit is composed of 2 deoxyribose sugars, 2 phosphates and 2 nitrogen bases. Here…

Answered: 1) Use the DNA origami model to…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Post lab questions 1) The approximate length of a DNA double helix is around 0.34nm per base pair. If the extracted DNA was in the form of a single double helix, calculate how long it would be in meters. (1nm = = 10-⁹m) Clear All Formatting 2) How long would it be in miles? (1mile = 1609.34m) 3) Is your double helix longer than the diameter of the Earth? 4) Is your double helix longer than the circumference of the Earth?
1). What is the concentration of a solution of DNA with an OD260 of 0.3? ----I got 15 micrograms/mL (50 x 0.3) 2). How many microliters of this solution of DNA would you need if you wanted 1.5 micrograms?
****0 b D. The Meselson and Stahl experiments demonstrated the mechanism of DNA replication by studying the density of DNA of cells that were first grown in the presence of 15N and then in presence of 14N isotopes. The following Figure "a" shows the results from Meselson and Stahl's experiment of DNA separated by desity gradient centrigugation. The lighter DNA is on the left and the heavier DNA is found on the right in the figure below. The Figure "b" shows the spectrum of the heavier DNA peaking on the rightside and the lighter one pearking on the leftside. Briefly, explain how the semiconservative mode of DNA replication is supported by their experimental results and what they may have seen if the replication modes were conservative or dispersive. GENERATIONS L. in i 3' Media Topoisomerase |||||||||||| comment Collaborate Helicase Primase 3 5' Replication fork AMAT Single-strand binding proteins 3' RNA primer Format Docum 5'
To answer the prompts below, draw on a piece of paper or your tablet the chemical structure of the trinucleotide 5' - TCA 3', labeling the 5' and 3' ends. Opposite this structure, - draw the complementary trinucleotide to make a double- stranded DNA molecule. A. What is the complementary trinucleotide sequence from 5' to 3' (enter answer as nucleotides only with no spaces)? B. How many non-covalent hydrogen bonds stabilize this double-stranded DNA structure? C. How many covalent phosphate linkages stabilize this double-stranded DNA structure?
Can you please check my answer and make sure it is correct? Question: Why are hydrogen bonds so vital to the structure of DNA? Hydrogen bonds are important for DNA because they are the bonds between the bases and help DNA form into its double-stranded shape. These bases include adenine, thymine, guanine, and cytosine. Adenine forms two hydrogen bonds with thymine and guanine forms three hydrogen bonds with cytosine. The resulting double-strands are held together by hydrogen bonds which is an important characteristic for DNA. These weak bonds are stable, but can be separated when it is necessary. This process is necessary during DNA replication, when a duplicate copy of DNA is being made or during the denaturation step in PCR.
I need help with worksheet ! Thank you
11:29 Protein 6-10092015113530.pdf https:api.schoology.comv1attachment169963838... Name Class Date 2. How are enzymes involved in this process? 3. hаppens anzips"? 4. Why is it important that exact copies of DNA be made? 5. Suppose that a sequence of one DNA strand is T-A-C-A-A-C-G-T-G. What is the corresponding sequence on the other strand? E Concept Mapping The construction of and theory behind concept mapping are discussed on pages vil-ix in the front of this Study Guide. Read those pages carefully. Then consider the concepts presented in Section 7-1 and how you would organize them into a concept i page 74. Notice that the concept map has been started for you. Add the key Now look at the concept map for Chapter 7 on concepts you are important Secti When you have finished the chapter, you will have a completed concept map. 69 1 of 1
TAC-ATA-ACG-CGA-CAA-CTA-AAA-ACT Write the amino acid sequence of the protein that would be formed by translating this plece of DNA. You can use the three letter abbreviations for the amino acid in each box, do not use the name of the full amino acids, Use the abbrevlation of the amino adid exactly as Itswritten in the table (including the appropriate capltalizations For example it your answer for amino acid 1 is"Methionine" you would write MET in the box (not Met or met) Second Later UUU UUUC Phe UCU UAU Ser UAC UAA UAG Tyr UGU Cys U Leu UCA ucG UGC Stop uGA Beop UUG Step UGG Trp G Cuu C cuC CUA CUG CU Leu Coc CCA CG CAU Pro cGu CAC CAA CAG His CGC Arg CGA CGG 1st 3rd letter AUU A AUG AUA AUG ACU le AAU AAC AGU ACC Asn Ser U leBer ACC ACA Mct ACG The AAA AAG ACA Lys AGG Arg acu Val GCC GCA GCG GAU GAC GAA GAG GOU GGC GGA GGG Arp G GUC GUA GUG Ala Gly Glu Seovence of the protein, 1st amino acid. 2nd arnino acid: 3rd amino acid: 4th amino acid Sth amino acid: 6th amino acid: 7th amino…
Can you please help me on these questions. I don’t know the right answers.
After properly troubleshooting, you finally are able to visualize the gel. You obtained the image below. The ladder used is “Lambda Hind III”.Knowing that the ladder “Lambda Hind III” consists of six fragments (23130 bp, 9416 bp, 6557 bp, 4361 bp, 2322 bp, 2027 bp), properly label the bands in the gel image.
Match the following terms with their correct definition. The structure of double-standed DNA Hold the base pairs together and make up the rungs of the DNA double helix One strand of nucleotides running in the opposite direction of the other strand Consists of a phosphate, a sugar backbone, and a nitrogen base A-T and C-G base pairs Five-carbon sugar found in DNA Answer Complementary base pairs Answer Double helix Answer Antiparallel Answer Nucleotide Answer Hydrogen bonds Answer Deoxyribose sugar
https://youtu.be/8kK2zwjRV0M Describe the purpose or theme of the video. Question 2 What are the chemical components of a DNA nucleotide? Question 3 List three characteristics of the structure of the DNA molecule. Question 4 Suppose you have a 5'-AGAGTGCGTA-3' sequence on one strand of the DNA. What is the base sequence that should appear on the other complementary strand of the DNA?

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