1 Making contact F plasmid F pilus Chromosome - Origin of transfer Donor cell F* Recipient cell F The F pilus contacts the recipient F- celI. 2 Initiating transfer One strand is cut in the origin of transfer The pilus retracts and pulls the donor and recipient cells together. 3 Transferring DNA A single strand of the F plasmid is transferred to the recipient cell; its complement is synthesized as it enters that cell. The strand transferred by the donor is replaced, using the remaining strand as a template for DNA synthesis. Transfer complete F* cell F+ cell At the end of the transfer process, both the donor and recipient cells are Ft and synthesize the F pilus. FIGURE 8.22 Conjugation-F Plasmid Transfer
1 Making contact F plasmid F pilus Chromosome - Origin of transfer Donor cell F* Recipient cell F The F pilus contacts the recipient F- celI. 2 Initiating transfer One strand is cut in the origin of transfer The pilus retracts and pulls the donor and recipient cells together. 3 Transferring DNA A single strand of the F plasmid is transferred to the recipient cell; its complement is synthesized as it enters that cell. The strand transferred by the donor is replaced, using the remaining strand as a template for DNA synthesis. Transfer complete F* cell F+ cell At the end of the transfer process, both the donor and recipient cells are Ft and synthesize the F pilus. FIGURE 8.22 Conjugation-F Plasmid Transfer
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Question
How does the recipient cell change as a result of conjugation?
Transcribed Image Text:1 Making contact
F plasmid
F pilus
Chromosome
- Origin of
transfer
Donor cell F*
Recipient cell F
The F pilus contacts the recipient F- celI.
2 Initiating transfer
One strand is cut in
the origin of transfer
The pilus retracts and pulls the donor and recipient cells together.
3 Transferring DNA
A single strand of the F plasmid is transferred to the recipient cell;
its complement is synthesized as it enters that cell. The strand
transferred by the donor is replaced, using the remaining strand
as a template for DNA synthesis.
Transfer complete
F* cell
F+ cell
At the end of the transfer process, both the donor and recipient
cells are Ft and synthesize the F pilus.
FIGURE 8.22 Conjugation-F Plasmid Transfer
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