.A protein gives a single band on SDS gel electrophoresis, as shown in lanes 1 and 2 below. There is little if any effect from adding B-mercaptoethanol (BME) in the gel; if anything, the protein runs a little bit slower. When treated with the proteolytic enzyme thrombin and electrophoresis in the absence of BME, the pro- tein migrates a bit more rapidly (lane 3). But if BME is present, two much more rapidly migrating bands are found (lane 4). Explain these results in terms of a model for the protein. 1 2 3 4 - BME + BME - BME + BME migration
.A protein gives a single band on SDS gel electrophoresis, as shown in lanes 1 and 2 below. There is little if any effect from adding B-mercaptoethanol (BME) in the gel; if anything, the protein runs a little bit slower. When treated with the proteolytic enzyme thrombin and electrophoresis in the absence of BME, the pro- tein migrates a bit more rapidly (lane 3). But if BME is present, two much more rapidly migrating bands are found (lane 4). Explain these results in terms of a model for the protein. 1 2 3 4 - BME + BME - BME + BME migration
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question

Transcribed Image Text:.A protein gives a single band on SDS gel electrophoresis, as shown
in lanes 1 and 2 below. There is little if any effect from adding

Transcribed Image Text:B-mercaptoethanol (BME) in the gel; if anything, the protein runs a
little bit slower. When treated with the proteolytic enzyme thrombin
and electrophoresis in the absence of BME, the pro-
tein migrates a bit more rapidly (lane 3). But if BME is present, two
much more rapidly migrating bands are found (lane 4). Explain
these results in terms of a model for the protein.
1
2
3
4
- BME
+ BME
- BME
+ BME
migration
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