Lab Report_Hematology_ Introduction to Blood_Updated (1)

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Jan 9, 2024

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NAME: Ivy Skipper DATE: 11/28/2023 Lab Report Hematology: Introduction to Blood This lab report is for you to reflect on what you completed and learned in this simulation, and to practice your written scientific communication skills. Include enough detail to clearly describe the lab experience to someone who understands the concepts but has not participated in the simulation. Use correct scientific terminology whenever applicable. Sections 1. Describe the overall objective and make a hypothesis 2. Introduce relevant background knowledge on this topic 3. Summarize the steps taken in the simulation 4. Explain any obtained results 5. Discuss the conclusions and implications 1 Copyright Labster ApS 2021 All Rights Reserved
1. Describe the overall objective and make a hypothesis. What is the overall purpose of the experiments or activities? Make a hypothesis. Recall that the hypothesis is a claim that answers a question or makes a prediction about an event that has not yet occurred. Hint: The purpose is often stated in the welcome message of the simulation (as shown in the example image below). For your entry make sure to include the following: Include one statement that describes the overall objective in your own words. Include one statement that describes the hypothesis. We will analyze the blood of three different patients. This process will entail collection, dissection, and distinguishing anomalies in the blood through testing. We will then observe the collected data to form a diagnosis so that the patients may receive suitable treatment. My hypothesis is that all three patients will have some type of disease or disorder. 2 Copyright Labster ApS 2021 All Rights Reserved
2. Introduce relevant background knowledge on this topic. What have you learned in class or researched on your own that would help prepare for this simulation? Hint: You can review the “THEORY” section in the simulation (as shown in the example image below) For your entry make sure to include the following: Include at least three important details from the lab “Theory” tab, the Lab Manual, or other resources, in your lab report that supports the simulation activity. Different types of blood disorders can affect people, causing conditions like anemia, lymphoma, myeloma, leukemia, and sickle cell anemia. Recognizable symptoms, such as excessive bleeding, swelling, and blood in stool or urine, can be indicators of potential blood disorders, prompting doctors to order blood tests to assess and diagnose the issue. Diagnostic tests for blood disorders include a range of procedures, such as the use of a Hematology Analyzer and microscopic examination of blood slides, allowing for the identification and visualization of abnormalities in various blood cell types. 3 Copyright Labster ApS 2021 All Rights Reserved
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4 Copyright Labster ApS 2021 All Rights Reserved
3. Summarize the steps taken in the simulation. Explain all steps you completed, including the equipment and techniques you used. Hint: You can use the “MISSION” tab in the LabPad as inspiration (as shown in the example image below). For your entry make sure to include the following: Document each step you completed, including the equipment and techniques you used. First, put on the proper lab attire including a lab coat and gloves. Then, gather blood samples from the patients using the purple capped blood tubes. Next, place the three tubes on the rack. Select the first tube, scan that tube, then use a pipette to draw out the blood sample. Place the sample on the slide. Place the slide under the hematology analyzer. Run the report and review the printed results. These steps are repeated for all three patients. Go to station 3. Put on new gloves. Then, select a tube from the rack, draw out the blood using a pipette. Place a drop of the patient’s blood on the slide. Smear the blood test on the slide using another clean slide. Dispose of the blank slide. Allow the slide with the blood to dry on the absorbing paper. Now remove it and place the slide in the giemsa stain for two minutes. Now remove the slide and wash it in the distilled water. Allow the slide to dry on the absorbing paper. Place the blood sample on the microscope stand. 5 Copyright Labster ApS 2021 All Rights Reserved
Apply the immersion oil to the slide. Repeat these steps for patients two and three. Notice Patient one shows signs of intracellular parasites. Notice patient two shows signs of abnormally shaped red blood cells. Notice patient three shows no signs of abnormalities. Review the patient charts at station two. Patient one is diagnosed with malaria. Patient two is diagnosed with sickle cell anemia. Patient three is diagnosed with hemophilia. Dispose of gloves and place the lab coat back on the rack. 6 Copyright Labster ApS 2021 All Rights Reserved
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4. Describe any obtained results. Explain all obtained results. Were these results expected or unexpected? Hint: You can use the “MEDIA” tab in the Lab Pad to revisit relevant images from the simulation (as shown in the example image below). For your entry make sure to include the following: Document the results from the simulation. Document whether the results were expected or unexpected, and briefly explain why. In the first stage of testing, patient one had an elevated white blood cell count and decreased platelet count, indicative of an infection. Patient two exhibited unusual red blood cells, suggesting a mild anemia. Patient three showed a normal blood count within the typical range for a healthy person. In the second stage of analysis, microscopic examination of blood samples revealed specific abnormalities. For patient one, intracellular parasites were identified on the blood slide, indicating a diagnosis of Malaria. Patient two had unusually elongated red blood cells were observed, confirming a diagnosis of Sickle Cell Anemia. Patient three’s blood tests revealed a potential for blood clotting issues and reduced levels of factors VIII and IX, pointing towards a possible diagnosis of hemophilia. This is further supported by a family history of hemophilia. These results were both expected and unexpected as I had expected all samples to show evidence of disease or disorder, however I did not 7 Copyright Labster ApS 2021 All Rights Reserved
expect these specific type of issues. 8 Copyright Labster ApS 2021 All Rights Reserved
5. Discuss the conclusions and implications. How do your results relate back to the original purpose and your hypothesis? Were there any systematic sources of error that could have affected the results? What did you learn? For your entry make sure to include the following: Include a statement that briefly summarizes how the results of the simulation relate back to your original purpose and hypothesis. Include a statement that details any systematic sources of error you identified that could have affected the results. If the lab did not have any systematic sources of error, include a statement that acknowledges the lack of error present in the lab. Include a statement about what you learned from the simulation. In this experiment, we successfully identified the blood disorders of three patients through a hematology test, enabling us to detect any variations in the complete blood count. The results were then further calibrated using a microscope to enhance accuracy. The simulation effectively demonstrated that comprehensive blood counts, combined with microscopic analysis, are crucial in identifying specific conditions such as Malaria, Sickle Cell Anemia, and hemophilia. This confirmed my hypothesis stating that all three samples had some type of disease or disorder. One possibility for error would be the sensitivity of the microscopic analysis, which could introduce variability in the identification of abnormalities. Additionally, variations in sample preparation and staining techniques may contribute to discrepancies in the results. While some degree of uncertainty exists, the overall accuracy of the results remains high, and the errors identified are inherent to the complexities of laboratory procedures. Through this simulation, we gained valuable insights into the diagnostic process for blood disorders. This experience enhances our understanding of the practical application of diagnostic techniques in hematology. 9 Copyright Labster ApS 2021 All Rights Reserved
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