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University of the Fraser Valley *
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1107L
Subject
Chemistry
Date
Jan 9, 2024
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Uploaded by advikaramesh
Introduction
The
market
for
dietary
supplements
has
been
growing
exponentially
as
the
desire
for
a
fit,
healthy
body
grows
in
society.
However,
the
weight
loss
industry
has
become
a
2.1-billion-dollar
industry
[1],
and
as
a
result
of
the
money
in
the
industry,
has
several
products
out
with
claims
that
it
cannot
satisfy
[2].
This
experiment
tests
the
validity
of
several
supplements’
claims:
if
they
provide
high
levels
of
protein.
The
experiment
aims
to
see
if
these
supplements
truly
do
contain
as
much
protein
as
they
claim,
as
well
as
seeing
how
much
fillers,
like
sugar
and
starch,
are
inside
of
these
supplements.
This
information
will
be
able
to
inform
fitness
aficionados
on
which
supplements
are
harming
them
versus
helping
them.
The
experiment
was
conducted
inside
of
a
lab
at
UGA,
where
qualitative
analysis
was
used
to
test
the
validity
of
the
supplements.
Methods
A
test
tube
with
2.5
mL
of
water
was
filled
with
0.5
mL
of
Lugol’s
reagent.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
yellowish-orange
tint
to
the
water
was
noticed
to
ensure
that
the
negative
control
was
set.
A
test
tube
filled
with
2.5
mL
of
stock
starch
solution
(0.1
g/L
starch)
had
0.5
mL
of
Lugol’s
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
bluish-black
tint
to
the
water
was
noticed
to
ensure
that
the
positive
control
was
set.
A
test
tube
filled
with
2.5
mL
of
Supplement
#1
had
0.5
mL
of
Lugol’s
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
color
change
was
noticed
and
recorded.
The
process
that
Supplement
#1
went
through
was
then
repeated
with
Supplement
#2
and
#3.
A
test
tube
with
2.5
mL
of
water
was
filled
with
0.5
mL
of
Benedict’s
reagent.
The
test
tube
was
covered
with
Parafilm,
mixed
and
then
heated
up
to
75°C
for
5
minutes
until
a
blue
tint
to
the
water
was
noticed
to
ensure
that
the
negative
control
was
set.
A
test
tube
filled
with
2.5
mL
of
stock
sugar
solution
(20
g/L
glucose)
had
0.5
mL
of
Benedict’s
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm,
mixed
and
then
heated
up
to
75°C
for
5
minutes
until
a
reddish-
brown
tint
to
the
water
was
noticed
to
ensure
that
the
positive
control
was
set.
A
test
tube
filled
with
2.5
mL
of
Supplement
#1
had
0.5
mL
of
Benedict’s
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm,
mixed
and
then
heated
up
to
75°C
for
5
minutes
until
a
color
change
was
noticed
and
recorded.
The
process
that
Supplement
#1
went
through
was
then
repeated
with
Supplement
#2
and
#3.
A
test
tube
with
2.5
mL
of
water
was
filled
with
1.0
mL
of
Biuret
reagent.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
blue
tint
to
the
water
was
noticed
to
ensure
that
the
control
was
set.
A
test
tube
filled
with
2.5
mL
of
stock
protein
solution
(5
g/L
albumin)
had
1.0
mL
of
Biuret
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
purple
tint
to
the
water
was
noticed
to
ensure
that
the
positive
control
was
set.
A
test
tube
filled
with
2.5
mL
of
Supplement
#1
had
1.0
mL
of
Biuret
reagent
added
into
it.
The
test
tube
was
covered
with
Parafilm
and
mixed
until
a
color
change
was
noticed
and
recorded.
The
process
that
Supplement
#1
went
through
was
then
repeated
with
Supplement
#2
and
#3.
Findings
Figure
1:
(Colorimetric
assay
with
Benedict’s
reagent)
(In
order:
Negative
Control,
Positive
Control,
Supplement
#1,
Supplement
#2,
Supplement
#3)
Figure
2
(Colorimetric
assay
with
Lugol’s
reagent)
(In
order:
Negative
Control,
Positive
Control,
Supplement
#1,
Supplement
#2,
Supplement
#3)
Your preview ends here
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Figure
3
(Colorimetric
assay
with
Biuret’s
reagent)
(In
order:
Negative
Control,
Positive
Control,
Supplement
#1,
Supplement
#2,
Supplement
#3)
These
were
the
findings
of
our
study.
The
photos
of
Benedict’s
reagent
test,
Lugol’s
reagent
test,
and
Biuret
reagent
test,
respectively,
are
displayed
above.
For
the
Benedict’s
test,
the
light
blue
indicates
that
there
is
little
to
no
sugar;
the
darker
that
the
red
is,
the
more
sugar
that
is
present.
These
results
indicate
that
all
of
the
supplements
have
some
amount
of
sugar
in
it.
Supplement
#2
had
much
less
sugar
than
Supplement
#1,
and
Supplement
#1
had
less
sugar
than
Supplement
#3.
In
the
Lugol’s
test,
a
yellowish-orange
color
indicates
a
lack
of
starch,
whereas
the
darker
the
color
is,
the
more
starch
there
is
in
the
solution.
In
terms
of
starch,
all
of
the
supplements
seem
to
also
contain
some
amounts
of
starch.
However,
it
seems
that
Supplement
#2
had
less
than
Supplement
#3,
which
had
much
less
than
Supplement
#1.
In
the
Biuret’s
test,
a
light
blue
indicates
the
absence
of
protein,
where
a
darker
blue
represents
more
protein.
The
results
for
protein
seemed
to
marginally
indicate
that
Supplement
#3
did
not
have
any
protein.
In
terms
of
the
supplements
that
did,
supplement
#1
had
the
least,
while
Supplement
#2
had
the
most.
Discussion/Conclusion
Because
Supplement
#2
had
the
least
amount
of
starch
and
sugar
while
having
the
most
protein,
Supplement
#2
would
be
the
one
that
the
fitness
club
should
recommend
to
its
constituents.
Supplement
#1
also
seems
to
come
close
in
terms
of
having
a
similar
amount
of
protein
as
well
as
a
little
less
sugar.
As
a
best-case
scenario,
the
experiment
looks
for
no
sugar
or
starch,
but
all
of
these
supplements
contain
sugar
and
starch
as
fillers.
With
the
parameters
that
the
supplement
should
have
no
fillers,
all
of
these
supplements
fail.
Works
Cited
1.
Arace
of
results.
Nutr
Bus
J
2016;21:1,
3-7.
2.
U.S.
Food
and
Drug
Administration.
Tainted
Weight
I.oss
Products.
2017.
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Recommended textbooks for you
- Chemistry: The Molecular ScienceChemistryISBN:9781285199047Author:John W. Moore, Conrad L. StanitskiPublisher:Cengage LearningChemistry In FocusChemistryISBN:9781305084476Author:Tro, Nivaldo J., Neu, Don.Publisher:Cengage Learning
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ISBN:9781285199047
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