Food Dyes Analysis of Commercial Products Part 2 (1)
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Chemistry
Date
Apr 3, 2024
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Food Dyes Analysis of Commercial Products Part 2
CHM2045L.017S24
Mar 28, 2024
Taylor Tran
Annabelle Martin
Sophia Bates
Maadh Alshaaili
Objective:
The purpose of this experiment is to understand and apply the principles of spectrophotometry
for chemical analysis. We will also need to be able to explain the interaction of UV-visible
radiation and molecules in commercial products. Creating and understanding a calibration curve
is also required. Alongside the calibration curve, understanding the electromagnetic spectrum,
light wavelength and color, UV-Visible absorption spectroscopy, spectrophotometer,
concentration of solutions, and dilution will also be needed.
Materials:
●
25mL volumetric flask
●
100mL beaker
●
7mL Graduated Pipettes
●
10mL Blue Powerade
●
10mL Loco Mango Monster Energy
●
10 mL Blue Gatorade
●
10 mL Fruit Punch Gatorade
●
Pipette pumps
●
Cuvettes
●
Pasco Scientific Vis-Spectrophotometer
Chemicals/Hazards:
Chemical Name
Chemical
Formula
Molecular
Formula Weight
Physical
Appearance
Specific Hazards
Dihydrogen
monoxide
H
2
O
18.01528 g/mol
Clear liquid
N/A
Red #40
C
18
H
14
N
2
Na
2
O
8
S
2
498.44 g/mol
Dark Red
Powder
May cause eye
irritation.
May cause
gastrointestinal
discomfort.
May cause
irritation to the
respiratory tract.
May cause
irritation to skin.
Blue #1
C
37
H
34
N
2
O
9
S
3
Na
2
792.86 g/mol
Dark violet
Powder
May cause eye
irritation.
May cause
gastrointestinal
discomfort.
May cause
irritation to the
respiratory tract.
May cause
irritation to skin.
Methods/Procedures:
1. Choose TWO food dyes that you want to work on from the list below:
- Blue #1
- Red #40
2. Choose a LIQUID commercial food product that contains the food dye you chose. Write the
name of the products here and the food dyes they contain. (Make sure to read the ingredient list
for the color and number associated with the food dye).
Blue Powerade: Blue #1
Blue Gatorade: Blue #1
Loco Mango Monster Energy: Red #40
Fruit Punch Gatorade: Red #40
3. What are the lambda max for the TWO food dyes you chose?
Blue #1: 598.4 nm
Red #40: 436 nm
4. What is an acceptable range for absorbance values?
Blue #1: 0.370 to 0.991
Red #40: 0.027 to 0.338
5. What will be used as the blank?
DI water filled cuvette
Calculations:
Calibration curve for blue dye:
Calibration curve for red dye:
Concentration:
𝑀
1
𝑉
1
=
𝑀
2
𝑉
2
Molarity:
𝑀
=
?𝑜?𝑒𝑠
/L
Beer’s Law:
𝐴
= ε
𝐶?
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References:
Laura Anderson; Jhon Figueroa; Vasiliki Lykourinou; et al. General Chemistry I Laboratory
Manual, 3rd Edition; 2024.
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