Short Lab Report Beers Law

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Florida State University *

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1020

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Chemistry

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Dec 6, 2023

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3

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Courtney Owens Tessa Moran Experient date: 9-13-22 Using Beer's Law to Find the Unknown Concentration of a Given Solution In this experiment, the unknown concentration of a solution was found using Beer’s Law, which states that the amount of energy absorbed by a solution is proportional to the concentration of solute in the solution. To apply this law, me and my partner used a stock solution and deionized water to create 3 different dilutions. These were Standard Solutions 2, 3, and 4. Then by using the dilution and concentration of solute (stock solution or Standard 1), we calculated the concentration of all 4 solutions. We also recorded the absorption of each solution, measured at 620 nm (the wavelength at which the max absorbance for the stock solution occurred. Data Calibration Standard Solution Concentration of Solution Volume of Solution Volume of Water Total Volume Concentration of Standard Solution Standard 2 7.75 x10^-6 8.00 mL 2.00 mL 10.0 mL 6.20x10^-6 Standard 3 7.75 x10^-6 5.00 mL 5.00 mL 10.0mL 3.875x10^-6 Standard 4 7.75 x10^-6 2.00 mL 2.00 mL 10.0 mL 1.55x10^-6 Calculating Concentration
Standard 2 (7.75x10^-6) x 8.00 divided by 10.0= 0.0000062 = 6.20x10^-6 Standard 3 (7.75x10^-6) x 5.00 divided by 10.0= 0.000003875= 3.875x10^-6 Standard 4 (7.75x10^-6) x 2.00 divided by 10.0=0.00000155= 1.55x10^-6 Calibration Graph Table Solution Absorbance Concentration Standard 1 (DI water) 0 0 Standard 2 0.696 6.20x10^-6 Standard 3 0.440 3.875x10^-6 Standard 4 0.163 1.55x10^-6 Standard 5 (stock solution) 0.860 7.75x10^-6 Commercial Product 0.485 4.20x10^-6
To calculate the concentrations of the standard solutions, we used the concentration of the stock solution and the dilution of the standard solution. The concentration of the commercial product was unknown. To find the absorbance of the commercial product, a solution with an unknown dilution, we used our existing data from solutions 1 through 5 by graphing a line of best fit(The dotted line in the graph shown above). The slope of this line showed the proportionality of absorbance to concentration for the solutions.
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