carbs and lipids handout Spring 22 (1)
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Qualitative Testing for Lipids, Carbohydrates, Starch and Protein
In this module, you will run a series of qualitative tests, detecting different classes of (macro) molecules in food samples.
The tests are:
Sudan Red
– a simple way to detect lipids.
Samples to be tested are spotted onto a filter paper and dried. The filter is then
submerged in a solution of Sudan Red, which will stain the lipid components in the sample spots.
A positive test will be indicated by
the presence of a red stained sample spot – the darker the red, the higher the lipid concentration in the spotted sample.
Benedict’s
– a simple way to detect reducing sugars.
Example: glucose. Requires the presence of an aldehyde group. Blue Cu++ ions
are reduced to reddish-brown Cu+ ions; a precipitate can also be formed.
Different colors indicate different concentrations of sugars
present.
Iodine
– a simple way to detect starch. As used in previous modules.
Biuret
– a simple way to detect protein. As used in previous modules.
Overview of testing:
Food sample
Sudan Red
Biuret
Iodine
Benedict’s
Olive oil
Test
Half and half
Test
Test
Test
Test
Potato Starch
Test
Test
Test
Test
Skim milk
Test
Test
Test
Test
water
Test
Test
Test
Test
Glucose
Test
Test
Sucrose
Test
Test
Specific instructions:
Part 1:
Each group will first perform the Sudan red test.
WEAR GLOVES.
Label the filter paper supplied with a pencil (
the Sudan Red dye is dissolved in alcohol which will remove ball point
pen ink), indicating group number and sample positions.
Spot a small amount of sample in separate spots, similar to the example shown below:
Dry under heat gun for a few minutes.
Submerge in solution of Sudan Red dye for 5 minutes: pour Sudan Red from tube into the petri dish over filter
paper
Half and half
Wearing gloves, remove filter into beaker of water to rinse for a few minutes.
Take filter out of water and let dry on paper towel.
Analyze sample staining, using “+++”, “+”, “-“ etc, as before.
Record your results in the table.
Part 2:
Benedict’s test:
Clearly label six 13x100 glass tubes with the name of the food sample
Mix 5 drops of sample with 5 drops of Benedict’s reagent
Incubate the tubes for 5 minutes in the 80 degrees C waterbath – HOT - careful!
Remove tubes to bench and let cool
Read and record the results, using the “+++”,
“++”,
“-“ scale as above
Biuret and Iodine test:
Clearly label twelve 13x100 glass tubes with (1) name of food sample and (2) testing reagent
Mix 5 drops of sample and 5 drops of either Iodine or Biuret reagent
Read and record the results, using the “+++”, “++”, “-“ scale as above
The goal is for each student to fill in result table #1 below.
Report:
Filled in result tables 1 and 2.
In a short paragraph, also describe the results in table 2.
Answer the following questions:
1.
What is the common feature for the Benedict’s and Biuret tests?
2.
Is the iodine-starch complex formation based on covalent bonds?
3.
Why can a disaccharide be a reducing sugar?
Food sample
Sudan Red
Iodine
Benedict’s
Olive oil
-
-
-
Half and half
++
-
+
Skim Milk
+
-
++
Potato Starch
-
-
-
water
-
-
-
Glucose
Table sugar
Setups:
Sudan red:
1 weigh boat
1 aliquot of Sudan Red
1 cut filter paper
1 beaker with water
Droppers
Food samples:
oliveoil, half and half, lactaid, almond milk, water
Benedict’s:
6 glass tubes 13x100
Benedict’s reagent:
5mL
80 degree waterbath with rack
Droppers
Samples: half and half,lactaid, almond milk, water, glucose, table sugar
Biuret:
4 glass tubes 13x100
Biuret reagent
5mL
Droppers
Iodine:
6 glass tubes 13x100
Iodine reagent 5 mL
droppers
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