carbs and lipids handout Spring 22 (1)

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Montclair State University *

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270

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Chemistry

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Dec 6, 2023

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docx

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3

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Qualitative Testing for Lipids, Carbohydrates, Starch and Protein In this module, you will run a series of qualitative tests, detecting different classes of (macro) molecules in food samples. The tests are: Sudan Red – a simple way to detect lipids. Samples to be tested are spotted onto a filter paper and dried. The filter is then submerged in a solution of Sudan Red, which will stain the lipid components in the sample spots. A positive test will be indicated by the presence of a red stained sample spot – the darker the red, the higher the lipid concentration in the spotted sample. Benedict’s – a simple way to detect reducing sugars. Example: glucose. Requires the presence of an aldehyde group. Blue Cu++ ions are reduced to reddish-brown Cu+ ions; a precipitate can also be formed. Different colors indicate different concentrations of sugars present. Iodine – a simple way to detect starch. As used in previous modules. Biuret – a simple way to detect protein. As used in previous modules. Overview of testing: Food sample Sudan Red Biuret Iodine Benedict’s Olive oil Test Half and half Test Test Test Test Potato Starch Test Test Test Test Skim milk Test Test Test Test water Test Test Test Test Glucose Test Test Sucrose Test Test Specific instructions: Part 1: Each group will first perform the Sudan red test. WEAR GLOVES. Label the filter paper supplied with a pencil ( the Sudan Red dye is dissolved in alcohol which will remove ball point pen ink), indicating group number and sample positions. Spot a small amount of sample in separate spots, similar to the example shown below: Dry under heat gun for a few minutes. Submerge in solution of Sudan Red dye for 5 minutes: pour Sudan Red from tube into the petri dish over filter paper Half and half
Wearing gloves, remove filter into beaker of water to rinse for a few minutes. Take filter out of water and let dry on paper towel. Analyze sample staining, using “+++”, “+”, “-“ etc, as before. Record your results in the table. Part 2: Benedict’s test: Clearly label six 13x100 glass tubes with the name of the food sample Mix 5 drops of sample with 5 drops of Benedict’s reagent Incubate the tubes for 5 minutes in the 80 degrees C waterbath – HOT - careful! Remove tubes to bench and let cool Read and record the results, using the “+++”, “++”, “-“ scale as above Biuret and Iodine test: Clearly label twelve 13x100 glass tubes with (1) name of food sample and (2) testing reagent Mix 5 drops of sample and 5 drops of either Iodine or Biuret reagent Read and record the results, using the “+++”, “++”, “-“ scale as above The goal is for each student to fill in result table #1 below. Report: Filled in result tables 1 and 2. In a short paragraph, also describe the results in table 2. Answer the following questions: 1. What is the common feature for the Benedict’s and Biuret tests? 2. Is the iodine-starch complex formation based on covalent bonds? 3. Why can a disaccharide be a reducing sugar? Food sample Sudan Red Iodine Benedict’s Olive oil - - - Half and half ++ - + Skim Milk + - ++ Potato Starch - - - water - - - Glucose Table sugar
Setups: Sudan red: 1 weigh boat 1 aliquot of Sudan Red 1 cut filter paper 1 beaker with water Droppers Food samples: oliveoil, half and half, lactaid, almond milk, water Benedict’s: 6 glass tubes 13x100 Benedict’s reagent: 5mL 80 degree waterbath with rack Droppers Samples: half and half,lactaid, almond milk, water, glucose, table sugar Biuret: 4 glass tubes 13x100 Biuret reagent 5mL Droppers Iodine: 6 glass tubes 13x100 Iodine reagent 5 mL droppers
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