Ahmed Chishti_Planning Project 3 (Team Submission)

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Michigan State University *

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162

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Chemistry

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Feb 20, 2024

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REFLECT ON YOUR TEAM CONTRACT Based on your team dynamics so far, is there anything that needs to be addressed or changed? Please list changes or aspects you believe your team is doing well, below. e | OV | tram dcsmxvuas @ R lare [go0d.! [We | work | | Lt R o il Al 40k .l,,.wu. B T I T e WU LR | take [sgce || ak e ouv | l WHAT QUESTION(S) ARE YOU BEING ASKED TO INVESTIGATE? - .. Based on the project scenario and goals, write down the Big-picture question(s) your team will investigate. (R | : 1 i i ! i i Proet { i | ; | | ! t b § ! | | d : | . = i | | j 7 i i i | , ] | i ! { { - ot 4 § 5 i t i i i { | | i i i e {755 i i | ] x ' .: E ,,il,_.,,, ,,Q.A ’, ,,,,, ,E, | §os By { EEE L 3 : i i i i i : { i | i i i ; i : ! i | i Include this page with your Planning submission. (Team Assignment) Food Dye Spectroscopy 107
photometer to measure UV and visible light absorbance PART 1: Use a spectro The UV-Vis spectrophotometers ava.ilable. in thls course measure the relative amount of light passing through liquid samples. For tI'us project, 1t 1s lmpf)rtant tl?at each member of your team can effectively use the Vernier SpectroVis+ Sensor. A techmgue guide on UV-Vis Spectroscopy and technology guide on Vernier Software & Devices are available in D2L for your reference. 4. Connect & Calibrate: Connect the SpectroVis+ sensor to your team’s dévice and calibrate the sensor. The dyes are prepared in RO/DI water so make sure to use that as your “blank” during calibration. 5. Measuring Samples: After calibration, collect a full spectrum of the provided dye solution. Then sketch (graph) the dye spectrum below. Include axes scales and labels. T Aosov iton TR R R U L T LA = S ey () | 6. Reading Spectra: How many peaks are present in your spectrum above? 7. What is the wavelength (Amax) for the highest absorbance peak? Does the dye greatly absorb the wavelength(s) of light you would expect a dye of its appearance to absorb? ,?\m 5 ,;ng i i 5“; & absovhs '.,b@m\n ommaq/ Include this page with your Planning submission. (Team Assignment) Food Dye Spectroscopy 108!
PART 2: Investigate the changes in UV and visible light absorbance as dye solution concentration increases. 8. Investigate the relationship between solution absorbance and concentration. Work as a team throughout the investigation, where each member completes measurements for one unique dye concentration. TIP: What quantitative methods did you use during the SCP planning to investigate the relationship between solution concentration and density? How can you apply those methods to this new scenario? We | lddgormimed | | tel | akedetn, tneochh graghg Dh Hwe spestva wiShn, We awm deromine Conceritiatoy Eomgh NN = My =Ny . wWe M e Hius eein W QMese WL gugn dye & the dihickions. | EERSRE o | Include this page with your Planning submission. (Team Assignment) Food Dye Spectroscopy o O
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e A =1 a0t Selwton la 2 421.7 T3S N gy, - 0 " Concentration Solution 9. Compile your team’ i Dilution | ¢ Dye (M) Absorbance to the left ik 1D ~axi -axi ' (x-axis) (it 10. Use Excel or other graphing software to 3 - . i n make a scaftterplot of density vs, . concentration (do not connect the data " 93z ints). ' S B0 LS points) . a. Include appropriate axes titles and p L x 1070 @@ \ Y units. e 11. Add a trend line and displ i | 294 and display the equation , LWMET & 1o and R? value on the chart/graph, TIP: 2 Refer to the Graphing guide in D2L. b - |, 040 12. Include a copy of your Excel graph T Al ylo ' below. —A . i | m; - - 1 - ¥ A B B X| 4 W A LW s 13. Examine the precision of your team’s data points relative to the applied linear regression. What can you do to improve your line-of-best-fit? TIP: An R? value of 0.98 or greater is ideal. N = 0.4% (\6: j‘f,%(}\w *\' 0azS 14. How does dye concentration relate to solution absorbance? What trend does your evidence > support? What molecular-level reasoning explains this trend? § ' [] / = we dugity pipodod . A dye/ : lessons e uader added. A 5 g < Include this page with your Planning submission. (Team Assignment) 11C
CONNECTING PRELIMINARY INVESTIGATION TO PROJECT GOALS ¢ How can UV-\{is spectroscopy be used to identify the food dyes present in a solution? If a beverage contained the dye used in the Preliminary Investigation, what key characteristics would you expect to observe in an absorbance spectrum of the beverage? D w Cor~ debrormime Yire concertiotcoe. = Mo&ow?ho\n, we_ coar e M ows c).\,\,U’v—tn& Yhe @wnocervtrediio i~ S ¢ How can dye concentration be determined from absorbance spectra? Use your team’s o.bservati(.)ns & data from Part 2 of the Preliminary Investigation to calculate the concentration (in molarity, M) of dye needed to produce a solution absorbance of 0.50 at the wavelength of maximum absorbance. TIP: Refer to the Standard Curves technique guide in D2L. we CMA~ ;?\y\% I~ 0NV odeso v pTilen~ Faow Grere we ‘wm-‘:«)»réx “’?\?“‘3 \Dbex\,S / Loy e el [ ISl e T s TRV I N T T ConcemIvad(ov Once you’ve calculated the concentration of dye needed, test your calculation by preparing a dilution of this dye concentration and measuring the sample’s absorbance spectrum. HINT: Use M1V =M, V.. Which variable will you need to solve for? | Aunicipated Absorbance (I | Mcstred Absbrpance 0.50 M, = L \ ViZ Include this page with your Planning submission. (Team Assignment) Food Dye Spectroscopy
'“:%Ey = hx“?fl iy »'u J‘Ww-eé a Q/\Ns‘r\ayx CEM 161 - N { C’w*\fl‘—w\j Tfid‘ GOAL 1: Experimentally determine the identity of the two food dyes present in your beverage. Consider the following in your design... ® What observations will you use to narrow down identities o What samples will you measure? How many of the two dyes present in your sample? samples will you measure? What order will * How will you use the SpectroVis+ to identify the two you measure the samples? dyes present in your sample? What data collection mode e What evidence will you gather to define the will you use? dyes present in your sample? * What solution will you use to calibrate the SpectroVis+? e We Wl Obsevue The whsovRMee & coubentid— Sv 0% fre duc. oo e L S ARRE T T ey ; R ode So— S . of e fi-o_ulé—s | I -y DIRG o culorore Spedmevat ® we W nwort. Kwe Coviocenkiodrion, oJon— BRIV I W W %(»c‘k‘ ve VS Wlsee \M\r@rfl\-&,\ o Include this page with your Planning submission. (Team Assignment)
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| PrOJECT entration of dye collection mode What range of absorbance values is acceptable? How will you create an assortment of * How many different measurements are needed to determine if your result is repeatable? * What evidence will you gather to mathematically determine the concentration of dye present in your sample? What calculations will you perform? concentrations? What strategy can you use regardless of the type of dye you end up using? e WM Py ue whsat Prug- ko ST | el Yot . s derrwine - vieevityate— PS D"G""‘e{r“" fi.e ."JDSQV\oM‘e-‘ ‘Cb'?'l Vel e We wse Pveviovs dade QOV*S Fov 'wg(sr | | e IR Wr€erSU4E AR S wAM ;«_. CQG"‘Q TR ¢ Bwc & b D e, AN il whc Include this page with your Planning submission. (Team Assignment)
pectroscopy 2Ny Ch ng SRR : Produce a 10 mL mock solution with the same Consider the Jollowing in your design... * How will you quantitatively prepare a 10 m[, mock e How will you calculate the average percent solution with the same color profile as your difference in solution absorption across all unknown sample? Your method mu. wavelengths of the visible spectrum (380— a guess-and-test approach, 700 nm). TIP; Use a spreadsheet/Excel. LA st be precise; not ] o - AR IR R AR S S III NI Include this page with your Planning submission. (Team Assignment) '177177777999979797992929202004¢ o - J-_— e - = = == =
119 g Wy G s R - §0 0 PR T Robid: s WI Q \(\7\’ , { s B ST R e T T 2 T e D SRRSO M T T }g\;.‘fl&’&-"‘@w: 0 | Course&Skction | CEM 161-__ . Hazards Handling / Disposal / Storage ‘5\?"*”\1 DM, Do et \ass Vie yloses . Nov-e_ Nowe s ki\\ SWR . st : Lorely c,udrs[\ovw...—- Poey doun dyvio Include this page with your Planning submission. (Team Assignment)
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PROJECT AR e —— ye Spectrw ' | Wogui~ Curs\h Ay Food D ,_ | (’W\f?\k}. et : Chhviste Team Members Avwned Oy S et 0 ll!lIIHIIIIIR 200N W E W RN NR YR e G- A EEE NN w~a Set g e £ 83 O Gx‘LmRS Lab Duration (hour) 3 V. s 5 s 2 B B 6 B & BEE H B HSS SN EEESEFENERBHEERFH LU U H AN EB N BN B , Describe who will complete different portions of your plan over the three-hour lab time. Each member is responsible for i J significant contribution of data collection. Include planned procedural steps, data to collect, and calculations where applicable” s Include this page with your Planning submission. (Team Assignment)