Experiment 5_ Extraction of Contaminants from Water

Table 1. Data on EDTA Standardization Weight of CaCO3 (g): 0.2003g Trial Volume of EDTA (mL) Molarity of EDTA (M) 1 8.60 2 8.50 8.55 Average Molarity of EDTA (M)

Table of chemicals and reagents with their physicochemical properties (name, molecular formula, molecular weight, pKa, melting point, boiling point, etc.) Write a short outline or a flow diagram of the procedure List and safety issues Read and understand the MSDS information for each chemical used in this practical Experimental procedure Please do not scratch the clear sides of the UV cuvettes as this will invalidate your results. Part 1: Preparation of a calibration curve A calibration curve should be constructed using at least five concentrations of the pure paracetamol standard supplied in the range 3-15 ug/mL as follows: Weigh about 150 mg of paracetamol accurately and transfer to a 200 mL volumetric flask. Add 50 mL of o.1 M NaOH and dilute to about 100 mL with deionised water (DI). Shake and make up to volume with DI water. Dilute 10 mL of the resulting solution to 100 mL (volumetric flask) with DI water (= stock solution). Prepare standard solutions containing 3, 6, 9, 12 and 15…

Color BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?

None

Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. X

Suzy Baker What three areas are affected by an "irritant"? (refer to ACS Safety in the Academic Chemistry Laboratories Vol. 1)    [use the answer format= ans 1, ans 2, ans 3]

Calculate the volume of NaOH used in each trial. Use sig figs.

best coulity solution.

A sample of rainwater was collected from four separate locations across theMetroplex. Each sample was measured once by two separate methods for the presence ofsulfate. Determine whether the two methods are equivalent with respect to giving thesame answer for [SO42-].

Please do it fast

12.8 A student is following a reaction (the conversion of compound A to Com- pound B) by TLC. Aliquots of the reaction mixture are taken and analyzed at time = 0, 10, 20, and 30 min. The TLC plate, developed in 90% hexanes-10% ethyl acetate, is shown below. (a) Which is the more polar compound, A or B? (b) Is the reaction complete at 10 min? 20 min? 30 min? (c) How can the identity of Compound B be verified by TLC? Compound A origin 0 10 20 30 Compound B

The concentration of green dye in green apple Gatorade was determined in a manner identical to experiment 3.The slope of the Beer lambast law plot (calibration curve) for this dye (FD & C Green 3 or fast green) was determined to be 53.7 L/g cm (assume the intercept of the beer lambast law plot is zero)what is the concentration,in units of g/L,of the green dye in the sample of Green Apple Gatorade with an absorbance of 0.369?In three significant figures

During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask.   0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL   1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)

Based on your value of Ksp1 what is the solubility of borax in the room temperature solution? Trial 1: 0.0141 T2: 0.015 AVG: 0.0139  Temp: 293.15K

You wash a separatory funnel with soap and water to ensure it is clean before extracting an aqueous solution with dicholormethane. You should take the time to make sure there are no drops of water left inside the separatory funnel. Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a True False

Pre-Lab Questions 1. Define a protein, and describe one important function of proteins in the human body. 2. The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (ug) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best fit line) to obtain the slope of the line. Determine the unknown protein content for a sample that shows an absorbance reading of b. С. 0.150.

We used tab water to extract caffeine from tea instead of using distilled water. How does it affect your result? Explain it scientifically. If you forgot to add anhydrous sodium sulfate, how does it affect your result? Explain it scientifically If you forgot to cap the TLC chamber, how does it affect your result? Explain it scientifically.

Absorbance 1.40 Beer's Law Plot 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0 10 20 30 40 50 Concentration (M x 105) D The slope for the line is 80.23. Use the Beer's Law plot provided to determine the concentration for a solution with absorbance = 0.5786. M