Table of chemicals and reagents with their physicochemical properties (name,molecular formula, molecular weight, pKa, melting point, boiling point, etc.)Write a short outline or a flow diagram of the procedureList and safety issuesRead and understand the MSDS information for each chemical used in this practicalExperimental procedurePlease do not scratch the clear sides of the UV cuvettes as this will invalidate your results.Part 1: Preparation of a calibration curveA calibration curve should be constructed using at least five concentrations of the pure paracetamol standardsupplied in the range 3-15 ug/mL as follows:Weigh about 150 mg of paracetamol accurately and transfer to a 200 mL volumetric flask. Add 50 mL of o.1 MNaOH and dilute to about 100 mL with deionised water (DI). Shake and make up to volume with DI water. Dilute10 mL of the resulting solution to 100 mL (volumetric flask) with DI water (= stock solution). Prepare standardsolutions containing 3, 6, 9, 12 and 15 ug/mL by mixing the appropriate volume of the stock solution with 10 mL0.1 M NAOH and diluting it to 100 mL (volumetric flask) with DI water. Read the absorbance values at 257 nm andconstruct a calibration curve using Cartesian graph paper.%3DAlsonhunceuglae(abebatu0, 19357レ60,0,7970,9969t AuseoyeAverayeAutottullet15 1.210-0.80,60.410.223101214161820Concentration8,60Part 2: Paracetamol assayThe following assay has been adapted from the British Pharmacopoiea (1999).ASSAY - "Weigh and powder 20 tablets. Add a quantity of the powder containing o.15 g of Paracetamol to 50 mlof o.1 M sodium hydroxide, dilute with 100 ml of water, shake for 15 minutes and add sufficient water to produce200 ml. Mix, filter and dilute 10 mL of the filtrate to 100 ml with water. Add 10 mL of the resulting solution to 10mL of o.1 M sodium hydroxide, dilute to 100 mL with water and measure the absorbance of the resulting solutionat the maximum at 257 nm"Calculate:The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambertlaw (A(1%, 1 cm) at 257 nm = 715)b) The percentage of stated content of paracetamol in the tabletsc) Compare and discuss the results from the two methodsAbsorbance

We will plot a calibration curve with absorbance as y axis and concentration as x axis which will…

Answered: Calculate: a) The average amount of…

Calculate: a) The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambert law (A(1%, 1 cm) at 257 nm = 715) b) The %3D percentage of stated content of paracet

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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toSave Paste Home Insert Draw Design Layout References Mailings Review View Help X US S Clipboard F 1 ge 2 of 4 1 4. Font Problem Set 5 - Chapter 5 Paragraph 2 A Styles 24 of 491 words Styles F Editing 3 Dictate Text Predictions: On Voice N: (g) + O2(g) → 2 NO (R) AH--180.5 kJ N2(g) + 3 H2(g) → 2 NHI(g) AH = -91.8 kJ 2 H2(g) + O2(g) → 2 H₂O (g) AH = -483.6 kJ Aidan Tomlinson AT 4 3. Calculate AH for the reaction 4 NO (g) + 6 H2O (g) →4 NH3(g) + 5 O: (g), from the following data. Editor Calculate AH for the reaction 2 Al (s) + 3 Ch (g) 2 AICI (s). given: 2 Al (s) + 6 HCl(aq) → 2 AICh (aq) + 3 H2(g) AH-1049. kJ HCI (g) → HCl (as) H: (g) + Ch (g) → 2 HCl (g) AICE (s) → AICia (94) AH--74.8 kJ AH-1845. kJ AH = -323. kl Focus F12 Reuse Files Editor Reuse Files FA 5 Editin 10:35 10/29/2 DELE
(a) Prove the relationship between the absorbance and concentration in Beer Lambert's law. What is the absorbance (A) of a sample if the transmittance (T) is 0.347?
Calculate the frequency of visible light having a wave length of 486 nm. Answer in s-1 to 3 sig figs
(i) Explain why Beer's Law breaks down when absorbance values are < 0.005. (ii) Explain the operation of a calcium source used in atomic absorbance spectroscopy. (iii) Explain why the calcium source described above will not operate for the quantification of magnesium.
Does a calibration curve alwasy compare concentration and absorbance? Or can it compare concentration and %T as well?
(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by. A = ID==ecL In=-= where lo is the incident light intensity, / is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and Lis the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (1) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹?
2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?
Q2-) Compare the sample strengths used in fluorescence spectroscopy and UV-Vis techniques.
) Calculate the change in enegy of the electron as it "falls" from n=4 to n= 2 using the values of Eq and E2 calculated in the prevous two problems. (AE= Ef-Ei)
(a) Calculate the energy difference between the two spin states of 1H and of 13C in a magnetic field of 6.8 T. 1H 4.0 1.19e-25 X J 13C 4.0 J (b) What is the precession frequency of a 1H nucleus at this magnetic field? Of a 13C nucleus? 1H 4.0 290 MHz 13C 4.0 73 MHz (c) At what magnetic field do protons precess at a frequency of 300. MHz? 4.0 7.04 T
(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?
Topic: Transmittance and absorbance, lambert-beer law Substance X has an absorbance maximum at 530 nm. 500 mg of this substance X are dissolved in one liter of water and the reading at 530 nm in a 0.10 cm thick cell, with the absorbance value equal to 0.215. They dissolve in water until make 250 mL a 150 mg quantity of a salt known to contain species X as the only absorbing species at 530 nm. The absorbance reading of the latter solution in a 1 cm thick cell is 0.750, what is the weight% of X in the salt?