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Calculate: a) The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambert law (A(1%, 1 cm) at 257 nm = 715) b) The %3D percentage of stated content of paracet

Calculate: a) The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambert law (A(1%, 1 cm) at 257 nm = 715) b) The %3D percentage of stated content of paracet

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Table of chemicals and reagents with their physicochemical properties (name, molecular formula, molecular weight, pKa, melting point, boiling point, etc.) Write a short outline or a flow diagram of the procedure List and safety issues Read and understand the MSDS information for each chemical used in this practical Experimental procedure Please do not scratch the clear sides of the UV cuvettes as this will invalidate your results. Part 1: Preparation of a calibration curve A calibration curve should be constructed using at least five concentrations of the pure paracetamol standard supplied in the range 3-15 ug/mL as follows: Weigh about 150 mg of paracetamol accurately and transfer to a 200 mL volumetric flask. Add 50 mL of o.1 M NaOH and dilute to about 100 mL with deionised water (DI). Shake and make up to volume with DI water. Dilute 10 mL of the resulting solution to 100 mL (volumetric flask) with DI water (= stock solution). Prepare standard solutions containing 3, 6, 9, 12 and 15 ug/mL by mixing the appropriate volume of the stock solution with 10 mL 0.1 M NAOH and diluting it to 100 mL (volumetric flask) with DI water. Read the absorbance values at 257 nm and construct a calibration curve using Cartesian graph paper. %3D Alsonhunce uglae (abebatu 0, 193 57レ 6 0, 0,797 0,996 9 t Auseoye Averaye Autot tullet 15
Transcribed Image Text:Table of chemicals and reagents with their physicochemical properties (name, molecular formula, molecular weight, pKa, melting point, boiling point, etc.) Write a short outline or a flow diagram of the procedure List and safety issues Read and understand the MSDS information for each chemical used in this practical Experimental procedure Please do not scratch the clear sides of the UV cuvettes as this will invalidate your results. Part 1: Preparation of a calibration curve A calibration curve should be constructed using at least five concentrations of the pure paracetamol standard supplied in the range 3-15 ug/mL as follows: Weigh about 150 mg of paracetamol accurately and transfer to a 200 mL volumetric flask. Add 50 mL of o.1 M NaOH and dilute to about 100 mL with deionised water (DI). Shake and make up to volume with DI water. Dilute 10 mL of the resulting solution to 100 mL (volumetric flask) with DI water (= stock solution). Prepare standard solutions containing 3, 6, 9, 12 and 15 ug/mL by mixing the appropriate volume of the stock solution with 10 mL 0.1 M NAOH and diluting it to 100 mL (volumetric flask) with DI water. Read the absorbance values at 257 nm and construct a calibration curve using Cartesian graph paper. %3D Alsonhunce uglae (abebatu 0, 193 57レ 6 0, 0,797 0,996 9 t Auseoye Averaye Autot tullet 15
1.2 10- 0.8 0,6 0.41 0.2 23 10 12 14 16 18 20 Concentration 8,60 Part 2: Paracetamol assay The following assay has been adapted from the British Pharmacopoiea (1999). ASSAY - "Weigh and powder 20 tablets. Add a quantity of the powder containing o.15 g of Paracetamol to 50 ml of o.1 M sodium hydroxide, dilute with 100 ml of water, shake for 15 minutes and add sufficient water to produce 200 ml. Mix, filter and dilute 10 mL of the filtrate to 100 ml with water. Add 10 mL of the resulting solution to 10 mL of o.1 M sodium hydroxide, dilute to 100 mL with water and measure the absorbance of the resulting solution at the maximum at 257 nm" Calculate: The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambert law (A(1%, 1 cm) at 257 nm = 715) b) The percentage of stated content of paracetamol in the tablets c) Compare and discuss the results from the two methods Absorbance
Transcribed Image Text:1.2 10- 0.8 0,6 0.41 0.2 23 10 12 14 16 18 20 Concentration 8,60 Part 2: Paracetamol assay The following assay has been adapted from the British Pharmacopoiea (1999). ASSAY - "Weigh and powder 20 tablets. Add a quantity of the powder containing o.15 g of Paracetamol to 50 ml of o.1 M sodium hydroxide, dilute with 100 ml of water, shake for 15 minutes and add sufficient water to produce 200 ml. Mix, filter and dilute 10 mL of the filtrate to 100 ml with water. Add 10 mL of the resulting solution to 10 mL of o.1 M sodium hydroxide, dilute to 100 mL with water and measure the absorbance of the resulting solution at the maximum at 257 nm" Calculate: The average amount of paracetamol per tablet using both the calibration curve and the Beer-Lambert law (A(1%, 1 cm) at 257 nm = 715) b) The percentage of stated content of paracetamol in the tablets c) Compare and discuss the results from the two methods Absorbance
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