Please help me to answer a question. I have 2 mg/mL stock solution and I need to make standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0 mg/mL. Water is used as diluent. How will make them by a serial dilution? As a result, I will need 50 uL of each sample for the next steps - so my method should produce in excess of 50 µL of each dilution. Please help me to calculate the mass of BSA in each sample too. Your help will be greatly appreciated.

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Please help me to answer a question. I have 2
mg/mL stock solution and I need to make
standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0
mg/mL. Water is used as diluent. How will I make
them by a serial dilution? As a result, I will need 50
µl of each sample for the next steps - so my
method should produce in excess of 50 µL of each
dilution. Please help me to calculate the mass of
BSA in each sample too. Your help will be greatly
appreciated.
Transcribed Image Text:Please help me to answer a question. I have 2 mg/mL stock solution and I need to make standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0 mg/mL. Water is used as diluent. How will I make them by a serial dilution? As a result, I will need 50 µl of each sample for the next steps - so my method should produce in excess of 50 µL of each dilution. Please help me to calculate the mass of BSA in each sample too. Your help will be greatly appreciated.
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