FLR 2 (1)

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Florida Gulf Coast University *

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2210

Subject

Chemistry

Date

Jun 11, 2024

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pdf

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Uploaded by DoctorIron11545

Identification of an Unknown Drug By a Thin Layer of Chromatography Experiment Performed: 1/23/2024 Introduction: In the following experience, we were tasked to identify an unknown sample and or substance by identifying the active ingredients present by using the Rf values determined by a thin layer of chromatography (TLC). By comparing the Rf values of pure chemicals (Aspirin, Acetaminophen, caffeine) to the Rf values to determine for the unknown sample of the identity of the unknown and said drug can be determined. Scheme/Equation

Equations and Calculations: Rf: distance traveled by the spot/ distance traveled by the solvent. Caffeine: 3.1 / 6 = 0.52 ASA: 4.1 / 6 = 0.68 AC: 3.5 / 6 = 0.58 U: 3.2 / 6 = 0.53 Sample Acetaminophen Caffeine Acetylsalicylic Acid Unknown #___ Rf 0.58 0.52 0.68 0.53 Safety/Hazards: Use gloves or forceps when working with silica gel on the TLC plates. When working with UV light, DO NOT look directly at it as it damages your eyes. Always wear your goggles. Procedure: Obtain the 3 known compounds (Caffine, Aspirin, and Acetaminophen) and the 1 NSAID drug sample (make sure to write the number of the unknown in the lab notebook). Find the Rf of the known compounds and of the unknown sample by using the TLC analysis and through observation of the spots. Prepare each sample, add a micro spatula of each sample into a vial of 2 ml of MeOH. Then gently draw on the base of the TLC plate with a pencil. Afterwards, spot the dissolved solution on the plate by using a capillary tube. Place the TLC plate in the chamber w/ 2-5 mL of ethylacetate. Make sure the TLC plate leaning on the side and not stuck on the side of the beaker. Allow the plate to develop until the solvent line is near the top. Remove the TLC plate with forecups, and draw in pencil a line along the solvent front so you can calculate the Rf, irradiated with the UV lamp. Circle the spots with a pencil. Then sketch the developed TLC plate

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