BIO250 Lab 2

pdf

School

Straighterline *

*We aren’t endorsed by this school

Course

MICROBIOLO

Subject

Biology

Date

Jan 9, 2024

Type

pdf

Pages

Uploaded by MegaRabbitPerson965

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Student Name: Click here to enter text. Access Code (located on the lid of your lab kit): Click here to enter text. Lab Report Format Expecta0ons U"lize college level grammar and professional forma4ng when comple"ng this worksheet. Submissions without proper forma4ng, all required photos or suﬃcient responses will be rejected. Pre-lab Ques>ons 1. This lab includes two experiments that each look at different aspects of culturing microorganisms onto growth media. What concepts does each experiment focus on? Why do you think these concepts are important to the field of microbiology? Click here to enter text. 2. In this lab, the experiments will allow or call for the use of four inoculaCon tools. List these tools and provide a scenario in which you would use each tool. ○ Click here to enter text. ○ Click here to enter text. ○ Click here to enter text.. ○ Click here to enter text. 3. Describe a piece of equipment used in biotech labs to extend the growth paJern of microbes. Focus parCcularly on those used with E. coli used to make human insulin. Click here to enter text. 4. You’re a physician trying to isolate bacterial colonies from the human gut in an aJempt to diagnose a gastrointesCnal infecCon. You streak your sample on a growth media containing glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7 . You incubate the plates in aerobic condi>ons at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in staCng that you had successfully cultured all the bacteria from your gut sample? Why or why not? Click here to enter text. EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION Introduc>on Ques>ons 1. Describe the objecCves of Experiment 1. State the goal of the experiment and the learning outcome that it is trying to convey to you as a microbiology student.

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Click here to enter text. 2. Proper asepCc technique is crucial to ensuring the growth of pure bacterial colonies. What will you do in this lab to demonstrate that you are using proper asepCc technique? Click here to enter text. In a laboratory seUng, what are three ways you can properly sterilize culturing equipment? ○ Click here to enter text. ○ Click here to enter text. ○ Click here to enter text. 3. What method of sterilizaCon will you use in this experiment? Click here to enter text. 4. What results do you expect to see with this experiment? For example, do you expect to see growth from all surfaces or just some? Are there parCcular strains you expect from the surfaces you swabbed? Click here to enter text.

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Data and Observa>ons In the table below, report your observed growth corresponding to each plate number and its corresponding source. Then provide a photo of your growth plates. Your data should have a quanCtaCve aspect to it, such as a count of the number of colonies that grew. Your data must also correspond to the photo you include for credit. Table 1: Colony Growth Provide a clear, high resoluCon photo of your plates aXer incubaCon with the lids removed. For credit on this lab, your photo must show the growth that is reported in your tabulated data and must also include your handwriJen name in the background. Plate Number Source Growth (Color, Amount, Shape, etc.) 1 Click here to enter text. Click here to enter text. 2 Click here to enter text. Click here to enter text. 3 Click here to enter text. Click here to enter text. 4 Click here to enter text. Click here to enter text. 5 Click here to enter text. Click here to enter text.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Sample 1 Sample 2 Sample 3 Control Airborne Contaminatio n

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Results and Discussion 1. Describe what morphological traits (i.e., size, shape, arrangement, color, margin, etc.) you observe from the growth on your plates. Be as specific as possible in the characterisCcs. Click here to enter text. 2. Based on the morphological qualiCes you observed on your plates, and using any resources available to you (i.e., a textbook or internet sources such as the CDC website), look up what bacteria you may have found on the surfaces you swabbed. Click here to enter text. 3. For the colonies hypothesized in QuesCon 4, are you surprised to find this type of microbe on this surface? Click here to enter text. 4. Looking at your control, did you perform proper asepCc technique or were your plates contaminated? If contaminaCon did occur, list the possible sources and how you can prevent contaminaCon in the future. Click here to enter text.

“ Lab 2 Culturing & Aseptic Technique BIO250L ” 5. Compare the colonies that grew on your swab plates to the Airborne ContaminaCon plate. Did any of your experiment plates grow colonies similar to your Airborne ContaminaCon plate? How would you describe the risk of airborne contaminaCon in your experiment? Click here to enter text. “

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

“ Lab 2 Culturing & Aseptic Technique BIO250L ” EXPERIMENT 2: BACTERIAL TRANSFER TO STAB TUBES AND AGAR PLATES Introduc>on Ques>ons 1. Describe the objecCves of Experiment 2. State the goal of the experiment and the learning outcome that it is trying to convey to you as a microbiology student. Click here to enter text. 2. What results do you expect to see in Experiment 2? Click here to enter text.

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Data and Observa>ons Record your observaCons in the tables below. Table 2: Ini>al Reserved Plate Colony Growth Observa>ons Table 3: Final Plate and Stab Tube Growth Observa>ons Plate Sample Appearance of the original colonies (morphology, etc.) 1 Input 2 Input 3 Input Sample Form Growth (Y/N) Same Appearance as Ini>al Plate (Y/N) Successful Transfer? (Y/N) 1 Plate Input Input Input 1 Stab Tube Input Input Input 2 Plate Input Input Input 2 Stab Tube Input Input Input 3 Plate Input Input Input 3 Stab Tube Input Input Input Control Plate Input Input Input Control Stab Tube Input Input Input

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Arrange your plates and tubes as shown below. Remove the lid from the plate and place the stab tube next to the matching plate. Then take a high resoluCon photo and insert it below with your handwriJen name in the background. For credit on this lab, the growth that you report in the above table must correspond to the photo below, and the photo must be of a high enough resoluCon to assess the reported data. Plate/Tube #1 Plate/Tube #2 Plate/Tube #3 Control

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

“ Lab 2 Culturing & Aseptic Technique BIO250L ” Results and Discussion 1. Review your data and determine whether or not the growth on your transfer plates have the same colony morphology (i.e., size, shape, arrangement, color, margin, etc.) as your original sample. Discuss this below. Click here to enter text. 2. If there was any difference in colony morphology, what do you think could have caused this? Click here to enter text.

“ Lab 2 Culturing & Aseptic Technique BIO250L ” 3. Imagine you are performing an experiment that requires the transfer of cells to different plates which will grow bacteria over an extended period of Cme (days or weeks). Describe why an unsuccessful transfer or growth with a different appearance from the iniCal plates would be a problem in this scenario. Click here to enter text. 4. Do some research and describe two or three scenarios in which it would be preferable to use a stab tube vs. a growth plate. ( Hint : What do bacteria use to help them move? Can moClity be used to help idenCfy many medically important pathogenic bacteria such as the Enterobacteriaceae family of bacteria?) Click here to enter text.

BIO250 Lab 2

Related Documents