BIO250 Lab 4

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Lab 4 Selective Media & Agar BIO250L ” Student Name: Click here to enter text . Access Code (located on the lid of your lab kit): Click here to enter text . Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet . Submissions without proper formatting, all required photos or sufficient responses will be rejected . Pre-lab Questions 1. In this lab, you will conduct three different experiments that each cover the topic of selective media and agar. How are these experiments different from one another? Explain how the different media in each experiment are utilized in the context of selective vs differential media. Click here to enter text. 2. What is the difference between chemically defined and chemically complex media? Give an example (a clinical or environmental research application) of when you would use each type of medium to culture different microorganisms. Click here to enter text. 3. Why do we grow microorganisms on general growth media first, before inoculating differential media? Click here to enter text. 4. What changes might you see on a differential plate that helps identify the bacteria? Click here to enter text.
Lab 4 Selective Media & Agar BIO250L ” EXPERIMENT 1: BIOPROSPECTING FOR STARCH DEGRADING BACTERIA Introductory Questions 1. What are the objectives of Experiment 1? What media will you use? How does this media pertain to the topic of selectivity? Click here to enter text. 2. What results do you expect to see in Experiment 1 if your selected colony is amylase-producing? Click here to enter text. 3. What are some other potential sources of starch-degrading bacteria? Click here to enter text. 4. Why did you perform Step 10, under the Prepare Stock Solution section, in this experiment? Click here to enter text. 5. What kind of media did you use initially, and why? Click here to enter text. 6. What is the purpose of the final section of this experiment? Click here to enter text .
Lab 4 Selective Media & Agar BIO250L ” Data and Observations In the table below, report your observed growth corresponding to each plate. Denote whether there was growth on the starch plate, whether or not clear areas formed around the colonies that grew . Table 1: Starch Agar and Gram Iodine Results Sample Growth on Starch Agar? (Yes/N ( Clear area around colonies? (Y/N ( A Input Input B Input Input C Input Input D Input Input Provide a clear, high resolution photo of your plates after incubation with the lids removed. For credit on this lab, your photo must show the growth that is reported in your tabulated data, must depict the correct media with the iodine added and must also include your handwritten name in the background . Plate A Plate B Plate C Plate D
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Lab 4 Selective Media & Agar BIO250L ”
Lab 4 Selective Media & Agar BIO250L ” Results and Discussion 1. Describe the results of your starch test. Click here to enter text. 2. Do the plates contain starch degrading bacteria? How do you know? Click here to enter text. EXPERIMENT 2: SELECTION AND DIFFERENTIATION OF GRAM POSITIVE BACTERIA Introductory Questions 1. What are the objectives of Experiment 2? What media will you use? How does this media pertain to the topic of selectivity? Click here to enter text. 2. What results do you expect to see from Experiment 2 if the microbes are non-fermenters? What if they are fermenters? Click here to enter text. 3. What chemical in MSA confers selectivity? How? Click here to enter text. 4. What component(s) makes MSA differential? How? Click here to enter text. 5. Why are the nutrient agar plates used in this experiment? Click here to enter text.
Lab 4 Selective Media & Agar BIO250L ” Data and Observations In the table below, report the growth that was observed on each of the plates and their corresponding source. Denote whether there was growth on the nutrient plate and MSA plates, and then denote the color of the MSA around the colonies. Also denote any other pertinent observations . T able 2: MSA Growth Observations Surface Tested Growth (Y/N) – Nutrient Agar Growth (Y/N) – MSA Agar MSA color around colonies (Red/Yellow ( Other Observations Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Input Provide a clear, high resolution photo of your plates after incubation with the lids removed. For credit on this lab, your photo must show the growth and color that is reported in your tabulated data and must also include your handwritten name in the background .
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Lab 4 Selective Media & Agar BIO250L ” Results and Discussion Skin/Nose MSA agar Surface/Control Nutrient Agar Surface/Control MSA Agar Skin/Nose Nutrient agar
Lab 4 Selective Media & Agar BIO250L ” 1. Review the photo of your agar plates. Describe the color around the colonies. Did any of the colonies change the color of the MSA? Click here to enter text. 2. What does the color of the agar tell you about the bacteria taken from each area of your body? Click here to enter text. EXPERIMENT 3: SELECTION AND DIFFERENTIATION OF GRAM-NEGATIVE BACTERIA Introductory Questions 1. What are the objectives of Experiment 3? What media will you use? How does this media pertain to the topic of selectivity? Click here to enter text. 2. What results do you expect from Experiment 3 if your growth ferments lactose? Click here to enter text. 3. W hat types of bacteria are inhibited on MacConkey agar? What ingredient(s) in MacConkey agar selects against those bacteria? Click here to enter text. 4. What ingredient(s) makes MacConkey agar differential? Click here to enter text. 5. Look up the formula for MacConkey agar either in a microbiology textbook or online . Is this a chemically defined or a chemically complex media? Why is that important? Click here to enter text. Data and Observations In the table below, report the growth that was observed on each of the plates. Also denote
Lab 4 Selective Media & Agar BIO250L ” T able 3: MacConkey Agar Results Sample Growth? (Yes or No ( Colony Color (Clear or Red ( Input Input Input Input Input Input Input Input Input Provide a clear, high resolution photo of your plates after incubation with the lids removed. For credit on this lab, your photo must show the growth and color that is reported in your tabulated data and must also include your handwritten name in the background . Plate 1 Plate 2 Plate 3
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Lab 4 Selective Media & Agar BIO250L ” Results and Discussion 1. Describe the growth you observed on the MacConkey Agar. Click here to enter text. 2. For each plate, provide an analysis of the results. What does the color of the colonies for each of the samples mean regarding the interpretation of the results? Click here to enter text.

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