BIO250 Lab 5

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L ” Student Name: Click here to enter text . Access Code (located on the lid of your lab kit): Click here to enter text . Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet . Submissions without proper formatting, all required photos or sufficient responses will be rejected . Pre-lab Questions 1. What is a model organism, and why is Saccharomyces cerevisiae so often used as one? Click here to enter text. 2. Research and discuss the properties of mold that make them sometimes beneficial to humans, and sometimes potentially harmful. Click here to enter text. 1 . The lab guide discussed “ acellular ” organisms. What characteristics classify them as acellular? Would you consider these living organisms ? Click here to enter text . 2 . D o some research and describe an acellular organism other than the two types discussed in the lab materials . Click here to enter text .

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L ” EXPERIMENT 1: MOLD GROWTH ON BREAD AND FRUIT Introductory Questions 1. What is the objective of Experiment 1? What outcomes do you expect to observe from this experiment? Click here to enter text. 2. In previous experiments, we have utilized agar to grow microorganisms. Why do you think we are utilizing bread and apples in this experiment? Why would agar not be an appropriate choice? Click here to enter text. 3. What was the purpose of examining three pieces of bread and three pieces of apple in each treatment? Click here to enter text. Data and Observations Record your observed mold growth in the table below. The data you include should be quantitative, rather than qualitative, such as the number of mold colonies denoted, or a percentage of the bread or apple covered in growth . Table 1: Mold Growth on Bread and Apple Samples Condition Day of First Visible Growth Extent of Growth on Bread (Mean ( Extent of Growth on Apple (Mean ( Bright, Control Input Input Input Bright, Water Input Input Input Bright, Sugar Input Input Input Bright, Lemon Input Input Input Bright, Vinegar Input Input Input Dark, Control Input Input Input Dark, Water Input Input Input

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L ” Results and Discussion 1. Which condition produced the most mold growth? The least? The fastest? The slowest? Click here to enter text. 2. Based on your results, what conditions are most favorable for mold growth on bread? On apple? Click here to enter text. 3. Would these conditions apply to all fungal growth? Click here to enter text. 4. Were there differences in how the mold mold on the bread vs the apple? If so, what do you think might account for these differences? Click here to enter text. 5. How would changing the type of bread (e.g. fresh from a bakery, no preservatives vs. prepackaged with preservatives) affect the results? Click here to enter text.

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L ” 6. How do you think changing the temperature at which the bags were incubated affected the results? Click here to enter text. 7. How would you test the predictions/hypotheses from Questions 5 and 6? Click here to enter text. 8. Look up the pH of lemon juice and vinegar. Based on your results and the results of your experiment for fungal growth, what can you conclude about the effect of pH on growth of mold? Click here to enter text. 9. Where did the mold come from? Click here to enter text.

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L ” Results and Discussion 1. Is Saccharomyces cerevisiae Gram-positive or Gram-negative? Research and describe the composition of yeast cell walls. How does the composition of yeast cell walls compare to the cell walls of Gram-positive or Gram-negative bacteria? Click here to enter text. 2. What information/structures were you able to glean from the methylene blue stain that you could not get from the Gram stain? Click here to enter text.