BIO250 Lab 7

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Lab 7 Microbial Genetics & Genetic Engineering BIO250L ” Student Name: Click here to enter text . Access Code (located on the lid of your lab kit): Click here to enter text . Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet . Submissions without proper formatting, all required photos or sufficient responses will be rejected . Pre-lab Questions 1. Why are the concepts in Lab 7 important in the field of microbiology? Click here to enter text. 2. Which DNA nitrogenous bases pair with each other? Click here to enter text. 3. Which bases are purines, and which are pyrimidines? Click here to enter text. 4. How is DNA information used to make proteins? What are the steps of this process, and what are the significant pieces of cell machinery that participate in this process? Click here to enter text. 5. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR changed biotechnology? Click here to enter text. 6. What are the three-nucleotide sequences that correspond to a specific amino acid called? Click here to enter text. 7. How could you take a known sequence of amino acids and use it to create an artificial gene? Hint: Think about this question in the context of Question 6, above. Click here to enter text.

Lab 7 Microbial Genetics & Genetic Engineering BIO250L ” EXPERIMENT 1: EXTRACTION OF DNA Introductory Questions 1. What is the purpose of the following reagents in the experiment? a. Salt (in the DNA extraction solution): Click here to enter text. b. Detergent (in the DNA extraction solution): Click here to enter text. c. Ethanol: Click here to enter text. 2. What do you expect to see when you perform Step 7 in this experiment? Click here to enter text. Data and Observations Insert a photo of your DNA collected on the wooden stir stick. Include your name and access code handwritten in the background of your photo. The photo must show the DNA as denoted in the lab, using the wooden dowel to remove it from the vial .

Lab 7 Microbial Genetics & Genetic Engineering BIO250L ” Results and Discussion 1. Was the DNA soluble in the aqueous solution or in the alcohol? Click here to enter text. 2. What else could have been present in the ethanol/aqueous interface? How could you have eliminated this? Click here to enter text. 3. What was the texture and consistency of the DNA when you collected it on the wooden stir stick? Click here to enter text. EXPERIMENT 2: CLONING A DNA FRAGMENT INTO A BACTERIALLY DERIVED PLASMID VECTOR Introductory Questions 1. What is the objective of Experiment 2, in simple terms? Click here to enter text. Data and Observations In the table below, list the number of base pairs in the longest length in base pairs for both types of DNA - foreign, and plasmid . Table 1: Fragment Lengths DNA Type Longest Length (in base pairs ( Foreign Click here to enter text . Plasmid Click here to enter text .

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Lab 7 Microbial Genetics & Genetic Engineering BIO250L ” Results and Discussion 1. What is the expected size of the plasmid plus the cut foreign DNA? Click here to enter text. 2. What type of ends do the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning? Click here to enter text. 3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does this enzyme work? Click here to enter text. 4. How would you clone a gene into a plasmid if there were no common restriction sites between the two DNA sequences? Click here to enter text.

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