Discussion 6 translation with key

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Bio 151 Rounds Discussion 6 Translation fall 2023 Discussion 6 Translation Team Portion Name(s) Discussion day and time: TA name: 1. Draw translation of the following mRNA:AUG CAU CGG AGU . Assume that it is translated in the first frame. Assume that the whole peptide has been translated. Pictured are the large and small subunits of the ribosome and the mRNA, along with the mRNA and four circles to represent the amino acids. Please add Include: the anticodcons in the P and E site. Label A, P, and E sites, 5’ and 3’, N and C terminus and amino acid name. Look up a codon chart online. (2 points) 1
Bio 151 Rounds Discussion 6 Translation fall 2023 2. Use the following mRNA sequence for the following. Assume the first three nucleotides are a codon and that sets the frame (it’s in the middle of the mRNA so doesn’t need a start codon): (2 point, 0.4 each) 5’ UGU AGU CGU UAA 3’ Write out the translated unmutated protein: C SR- cysteine serine arginine stop Give an example of each showing the mRNA ( please underline the change ) change and the new protein: Missense: change any amino acid except stop Nonsense: change any of first three to a stop codon Frameshift: 2
Bio 151 Rounds Discussion 6 Translation fall 2023 insert or delete 1 or 2 nucleotides not 3 Silent: 3. Translate the following sequence in all three frames. Start with the first nucleotide, the second nucleotide and the third. You should have three sequences. (2 point): 5’ ACAAUCCAGACG 3’ TIQT QSR NPD 3
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Bio 151 Rounds Discussion 6 Translation fall 2023 Discussion 6 Translation Individual Portion Name: Discussion day and time: TA name: 1..a. Using the codon chart on the separate sheet translate the following mRNA, assume AUG is the start codon (2 point) . If you started in the second frame how would the sequence be different? 5’-AUG UUU UAC AUU GC U UAC -3’ M FYIAY Methionine, phenylalanine, tyrosine , isoleucine, alanine, tyrosine, CFTLL Cysteine, phenylalanine, threonine, leucine, leucine Name the mutation caused by changing the underlined nucleotide in the sequence above in the following ways: b. Deleting it (1 point): frameshift c. Changing it to a C (1 point): Silen . 4

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