Discussion 11 enzyme kinetics and energetics F23 key

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Bio 151 Rounds Discussion 11 fall 2023 Discussion 11 energetics and enzymes Team Portion Names: TA: Discussion day/time: 1. On the chart to the right draw the time course for the following reaction ATP → ADP + P i . The chart should show the relative free energy of the reactants and the products (not the exact numbers but their relative magnitude – which is higher and which is lower). Label product, reactant, activation energy, and whether the reaction is exergonic or endergonic. (1 point) Two enzymes, smileyface hydrogenase (dots) (k m = 1.6 nM V max = 20 nM/s) and poutyface reductase (solid) (k m = 10 nM V max = 20 nM/s), can both convert the substrate pouty into the product smiley using NADH. Which of these statements are true? (A=true, B=False) (1 point each) 2. Poutyface reductase should work better (have a higher rate) than smileyface hydrogenase at low pouty concentrations <100 nM. B 1
Bio 151 Rounds Discussion 11 fall 2023 3. At very high concentrations of pouty, both enzymes will have the same rate. A 4. Consider the reaction ADP + P i → ATP (+30.5 kJ/mol). What reaction could “drive” this reaction by being coupled to it? (1 point) A. creatine + P i → creatine phosphate (+43.1 kJ/mol) B. glucose-6-phosphate → fructose-6-phosphate (+1.7 kJ/mol) C. phosphoenolyruvate → pyruvate + Pi (-61.9 kJ/mol) D. Fructose-1,6 bisphosphate → Fructose-6-phosphate + Pi (-16 kJ/mol) In the above reaction scheme which of the following are correct? (A=true, B=false) (0.5 each) 5. Ethanol reduces NAD + A 6. The conversion of ethanol to acetaldehyde is exergonic A 7. Draw a graph showing an enzyme with K m of 40 mM and a Vmax of 15 mM/s. Label axis (2 points). 2
Bio 151 Rounds Discussion 11 fall 2023 3
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Bio 151 Rounds Discussion 11 fall 2023 Discussion 11 energetics and enzymes Individual Portion Names: TA: Discussion day/time: Use the diagram at right for the next 2 questions. The data represents an enzyme that catalyzes the conversion of arachidonic acid to prostaglandin leading to inflammation and pain. It exists in three different tissues performing slightly different functions. (0.5 each) 1. Which version of the enzyme is most effective at a very low concentration? A 2. Which enzyme has the larger value Km? B In the reaction scheme at right which of the following are correct (A=true, B=false)? (0.5 points each) 3. Acetaldehyde reduces NAD + A 4. Acetic acid oxidizes NADH B Use the diagram at right to answer the following questions (1 point, 0.5 each). (A=true, B=false) 5. As drawn, the diagram shows an endergonic reaction. A 6. The reaction could be catalyzed by an enzyme with no coupling to an exergonic reaction B 4

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