Question 11 pts

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University of Florida *

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6459

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Biology

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Jan 9, 2024

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Question 11 pts Find the gene ID (also called locus tag) for protein 1 (UniProt ID: Q184Q8) and post it. CD630_30720 Flag question: Question 2 Question 21 pts Find the gene ID for protein 2 (UniProt ID: Q189Q8) and post it. CD630_07100 Flag question: Question 3 Question 32 pts Find the family protein 1 belongs to and give the family name (hint: it should begin with "DUF"). DUF523 Flag question: Question 4 Question 42 pts Explain in a few sentence how you found the family information and embed an image that supports your findings. I searched for the uniport id on uniport.Then I clicked on external links and clicked on the “Biocyc”.In biocyc, located at the bottom of the page is “Pfam In-family” and clicked on it. p Flag question: Question 5
Question 51 pts Protein 2 belongs to the same protein family as Protein 1 Group of answer choices True yes False Flag question: Question 6 Question 62 pts Do a multiple sequence alignment of the proteins given Here Download Hereand insert the resulting alignment in the response box. What strikes you about the residues that are conserved? The residues that are conserved are few ,there are many gaps. When Phylogeny.fr: "Advanced" Mode used the conserved positions is 4%. Flag question: Question 7 Question 72 pts Do a phylogenetic tree with the same set of proteins and insert the resulting tree it in the response box. You can use any platform to do the tree but if you use Phylogeny.fr use (http://phylogeny.lirmm.fr/phylo_cgi/index.cgiLinks to an external site.) the "step by step" and choose the less stringent conditions.
p Flag question: Question 8 Question 82 pts Find papers that describe the function of members of the protein family. Find the sequence (in Fasta format) of another protein within this family that has been experimentally validated and call it "VALIDATED_Protein3" in your fasta header. Add it to the protein list that was given in Q6 and redo the phylogenetic tree. Embed the new tree in the response box. p Flag question: Question 9 Question 92 pts After analyzing the tree, do you think Protein 1, Protein 2, or neither of them is/are isofunctional with Validated_Protein3. Justify you answer in a few sentences. Protein 2 is isofunctional with Validated_Protein3 . They emerged from a common ancestral node. Protein 2 and Validated_Protein3 are not duplicated.They were created by speciation (orthologs) which generally implies function remains the same
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