Fa 23 Microbial Lab Schedule - REVISED_-359802875

MCB 3020C: Microbial Biology Fall 2023 - REVISED Lab Schedule – subject to change Date Lab Activities Lab Manual Sections 8/23 lab safety & ubiquity of microbes & Winogradsky column introduction Section 2-1, 8-1, pg 1-7 8/25 ubiquity of microbes & microscope Section 2-1, 2-2, 3-1, 3-2, pg 181-184 8/30 Hurricane Idalia 9/1 aseptic technique Winogradsky Discussion post due Section 1-3, pg 27, Appendix C 9/6 simple stains Section 3-5, 3-6, pg 181-184 9/8 simple stain, negative stains, & pour plates Section 3-5, 3-6, 3-9, pg 181-184 9/13 quadrant streak plates & spread plates Section 1-4, Canvas Handout 9/15 Differential staining: Gram, Endospore and Acid Fast Section 3-7, 3-8, 3-10 9/20 Differential staining: Gram, Endospore and Acid Fast Section 3-7, 3-8, 3-10 9/22 Catch up & practice for hands on exam Microscope worksheet Due 9/27 Lab Written Exam 9/29 Hands On Exam: Gram Stain, Streak and Spread Plates 10/4 Quantification of bacteria: standard plate count - Meat experiment part 1 Section 6-1, Canvas Handout 10/6 Quantification of bacteria: standard plate count analysis - Meat experiment part 2 & Receive Unknown 1 Quantification of Bacteria Due Winogradsky Discussion post due Section 6-1, Canvas Handout

Date Lab Activities Lab Manual Sections 10/11 Aerotolerance (deeps, shakes, thioglycollate, gas pak) Section 2-6, 2-7, 2-8 10/13 Carbohydrate fermentation (oxidation-fermentation, MRVP) Meat Assignment due Section 5-2, 5-4, pg 286 10/18 Carbohydrate fermentation (phenol red broth, KIA) Aerotolerance Quiz Section 5-3, 5-21, pg 286 10/20 Carbohydrate fermentation (SIM, motility, citrate utilization) Section 5-9, 5-20, 5-28 10/25 Respiration & Metabolism (catalase, oxidase, nitrate reduction) Section 5-6, 5-7, 5-8 10/27 Bacterial Report Introduction Carbohydrate Quiz Section7-7, Canvas Handout 11/1 Selective & Differential media (Mannitol salt, MacConkey agar, EMB) Respiration/Metabolism Quiz Section 4-2, 4-4, 4-5, 4-6, pg 236 11/3 Differential Media (Blood agar, Columbia CNA, DNase) Winogradsky Discussion post due Section 5-25, canvas handout 11/8 Unknown 2 Introduction Receive unknown 2 11/10 NO LAB - Veteran’s Day 11/15 analysis of unknown Selective/Differential Media Quiz Bacterial Identification Report 1 11/17 analysis of unknown DNA sample prep 11/22 NO LAB – Thanksgiving 11/24 NO LAB - Thanksgiving 11/29 analysis of unknown 12/1 analysis of unknown Blast Day 12/6 analysis of unknown Winogradsky Column Mature Assignment due 12/8 Winogradsky Columns & analysis of unknown Bacterial Identification Report 2