Biochemical test reusults - online (1)

docx

School

Middlesex County College *

*We aren’t endorsed by this school

Course

110

Subject

Biology

Date

Jan 9, 2024

Type

docx

Pages

Uploaded by DeaconSalamanderPerson1017

Biochemical test results: Read the results that you have for Gram-positive and Gram-negative organisms. Gram Positive organism - Possible Gram +ve results: Results Catalase test: +ve, Coagulase test: -ve , MSA plate: The organism grew on the plate and the media turned yellow. Organism is : Staphylococcus aureus II. Results: Catalase test: +ve, Coagulase test: -ve , MSA plate: Off-white colored bacteria grew on the plate but the media of the plate did not change color, and remained pink. Organism is : Staphylococcus epidermidis III. Results: Catalase – Negative Bile Esculin test – positive with black precipitate Blood agar plate – No hemolysis; White colored colonies on the Blood agar plate Organism is : Enterococcus faecalis IV. Results: Catalase – Negative Bile Esculin test – Negative; No black coloration Blood agar plate – alpha hemolysis or partial hemolysis. Greenish brown color around the colonies on the Blood agar plate Organism is : Streptococcus agalactiae Gram-negative organisms: Non enteric 1. Results: Oxidase – Positive with a bluish-purple color

Nitrate Reduction test: Bubble in Durham tube; Nitrate is reduced to N2 gas. Cetrimide: Grows on cetrimide plate and produces a green pigment that fluoresces under UV light Organism is : Pseudomonas aeruginosa 2. Results Oxidase – Positive with a bluish-purple color Nitrate Reduction test: No Bubble in Durham tube; No color change after addition of nitrate reagents A and B . Red color after addition of zinc dust. The organism is negative for the nitrate reduction test. Cetrimide: No growth on cetrimide Organism is : Alcaligenes faecalis Gram-negative organism - Results for Enteric organism 1. Nitrate test - Look for a bubble in the Durham tube -- Bubble indicates conversion of Nitrate to N2 gas – Occurs with Pseudomonas If you do not see the bubble, add nitrate regent A and B (5 drops of each, mix after each addition). The red color indicates the reduction of nitrate to nitrite – All enteric organism converts nitrate to nitrite If you do not see the red color after the addition of nitrate A and B, add Zn dust. Mix. Wait. If the tube turns red after the addition of zinc, it is negative for the nitrate reduction test. Ex: Alcaligenes 2. SIMs test M – Motility – If the organism moves away from the point of insertion of the needle or the tube is cloudy, the organism is positive for Motility. Most of the organisms we use in the lab are motile. f the growth organism stays within the point of insertion of the needle, the organism is non- motile. Example Klebsiella pneumoniae

S reduction to H2S – black coloration is a positive result. Proteus is positive for s reduction. Citrate is weak +ve. Indole Production – Add Kovac’s reagent about 4 to 5 drops; the pink color at the top of the tube indicates that the organism converts tryptophan to indole. Proteus and Citrobacter give a positive result. 3. MRVP test: MR test : Take 1ml aliquot of the incubated broth for MRVP in a 5ml tube and add a few drops of methyl red reagent. The red color formation is +ve for the MR test. E. coli, Serratia, (and Citrobacter give a positive result. Proteus gives a weak positive result. VP test: Take 1ml aliquot of the incubated broth for MRVP in a 5ml tube and add 9 drops of VP A, mix and 5 drops of VP B and mix and wait for 30min. The red coloration on the top of the solution is +ve for the VP test. Enterobacter aerogenes and Serratia are positive for the VP test and negative for the MR test. 4. Citrate test: If the citrate tube turns royal blue color, the organism uses citrate as a carbon source and produces alkaline products which turn the media blue in color and is positive for citrate test. Enterobacter aerogenes, Serratia, and Citrobacter are positive for the citrate test If the media remains green in color, the organism is negative for the citrate test. E. coli and Proteus are negative for the citrate test. 5. Urease test: If the organism is positive for the urease test, it will convert urea to ammonia which will turn the media fuchsia pink color. You will get an orange color if the organism is weak-positive for the urease test. Proteus is positive for urease test. The media remains yellow for urease-negative organisms. Enterobacter aerogenes, Serratia, E.coli, and Citrobacter are negative for the urease test Follow the lab manual, PowerPoint or class notes to do the tests. Note down the results in your notebook. Compare your results with the results on the chart in the lab manual and identify the organism. Write the full Scientific name of the organism and underline or italicize the name Look for a complete match, at least most of the results should agree. If there is a discrepancy in a particular result, please give reasons for it in your report.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Use unknown reports in the modules for the submission.

Related Documents

exam-18-december-2009-questions.pdf

C888 Task 1.docx

`BIOL-Learning Journal Unit 2.docx

BIOL-Written Assignment Unit 1.docx

BIO-Written Assignment Unit 3.docx

BIO-Discussion Forum Unit 3.docx

The Effect of Temperature on an Enzyme Catalyzed Reaction - Student Handout.docx

study guide part 3 rollup.docx

Axial Skeleton Quiz #2.docx

AP 1 Test 1.docx

C728 Task 1.docx

C728 Task 2.1.docx

Recommended textbooks for you

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Biomedical Instrumentation Systems

Chemistry

ISBN:9781133478294

Author:Chatterjee

Publisher:Cengage

Microbiology for Surgical Technologists (MindTap ...

Biology

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Cengage Learning

Comprehensive Medical Assisting: Administrative a...

Nursing

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Cengage Learning

Complete Textbook Of Phlebotomy

Biology

ISBN:9781337464314

Author:Hoeltke

Publisher:Cengage

Basic Clinical Laboratory Techniques 6E

Biology

ISBN:9781133893943

Author:ESTRIDGE

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage