Restriction Digestion of E

2023-11-30, 12 : 24 PM Restriction Digestion of E. coli JM101 Utilizing Uncut, EcoR1, Bam…omponents, Followed by Gel Electrophoresis Separation. · Benchling Page 1 of 3 https://benchling.com/mpate445/f/lib_Z4eJ9ZSx-biology-2290g-protoco…is-separation-/view ? versionId=816768891&note=true&protocols=#print Restriction Digestion of E. coli JM101 Utilizing Uncut, EcoR1, BamHI, and E+B Components, Followed by Gel Electrophoresis Separation. Purpose : After being subjected to restriction digestion using di ff erent enzymes (Uncut, EcoR1, BamHI, and E+B), the resulting fragments of E.coli JM101 were separated using gel electrophoresis. 1. Work in partners .As a pair you will complete the following reactions 2. Get four 1.5 mL microcentrifuge tubes and label them “Uncut”, “EcoR1 ”, “BamHI”, “and “E +B” and your initials 3. Look at the four tables below and add the appropriate components to each tube: Component: Uncut Volume Autoclaved dH 2 O 6 μ L Loading Dye 2 μ L 250 ng Plasmid DNA. 2 μ L TOTAL 10 μ L A B 1 2 3 4 5 6 THURSDAY, 2023-11-30 Table1 Explore the Notebook

2023-11-30, 12 : 24 PM Restriction Digestion of E. coli JM101 Utilizing Uncut, EcoR1, Bam…omponents, Followed by Gel Electrophoresis Separation. · Benchling Page 2 of 3 https://benchling.com/mpate445/f/lib_Z4eJ9ZSx-biology-2290g-protoco…is-separation-/view ? versionId=816768891&note=true&protocols=#print Component: EcoRI Volume Autoclaved dH 2 O 4 μ L 5 x FastDigest Bu ff er with Loading Dye 2 μ L 250 ng Plasmid DNA. 2 μ L EcoRI 2 μ L TOTAL 10 μ L A B 1 2 3 4 5 6 Component: BamHI Volume Autoclaved dH 2 O 4 μ L 5 x FastDigest Bu ff er with Loading Dye 2 μ L 250 ng Plasmid DNA. 2 μ L BamHI 2 μ L TOTAL 10 μ L A B 1 2 3 4 5 6 Table2 Table3