Micropipetting and Spectrophotometry Quiz Guide

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 1/12 Prac 1 - Micropipetters and Spectrophotometry Started: 16 Mar at 19:45 Quiz instructions Answer the following questions based on the data you collected during your first practical. 0 pts Question 1 p What size pipette did you calibrate? 1000μL 1 word </> 0 pts Question 2 What was the upper volume setting you tested (in μL)?

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 2/12 p span 800μL 1 word </> 2 pts Question 3 What were the three masses you delivered on this higher setting (in mg)? 802.1mg 803.0 mg 803.9 mg

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 3/12 p 5 words </> 1 pts Question 4 p Based on the average mass delivered, and assuming the density of water is 1 g per mL, what is the percent deviation from the set volume? (i.e. the difference between this upper set volume and the delivered volume, as a percentage) Density =mass / volume V = 0.8030mg / 1gmL-1 = 0.8030mg / 1gmL-1 = 0.8030mL percent devia±on = 0.8000 / 0.8030 x 100% = 99.63% 23 words </> 0 pts Question 5 What was the lower volume setting you tested?

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3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 4/12 p 400μL 1 word </> 2 pts Question 6 What were the three masses you delivered on this lower setting? 400.1 mg 400.5 mg 400.8 mg

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 5/12 p 6 words </> 1 pts Question 7 p Based on the average mass delivered, and assuming the density of water is 1 g per mL, what is the percent deviation from the set volume? (i.e. the difference between this lower set volume and the delivered volume, as a percentage) Density =mass / volume V = 0.4004mg / 1gmL-1 = 0.4004mg / 1gmL-1 = 0.4004mL percent devia±on = 0.4000 / 0.4004 x 100% = 99.9% 23 words </> 1 pts Question 8 590 What wavelength setting (in nm) did you use for the spectrophotometry of bromophenol blue?

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 6/12 4 pts Question 9 Upload Your file has been successfully uploaded. Please upload an image of the standard curve you produced, with proper labeling, scale, and a line of best fit.  The standard curve.jpg 1 pts Question 10 p Based on your standard curve, what is the molar absorptivity of bromophenol blue? A = εlc y =mx A= y , c= x and ε = m x l ε = 0.0428 18 words </>

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3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 7/12 2 pts Question 11 p What absorbances did you record for your two unknown BPB replicates? 1. 0.589 2. 0.598 4 words </> 1 pts Question 12 Based on the average absorbance of the unknowns and the molar absorptivity for bromophenol blue you determined, what is the concentration of the unknown sample? Average absorbance of the unknowns = 0.5935 A = εlc 1

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 8/12 p A = εlc 0.5935 = 0.0428 x 1 x c c = 13.8668 μM 21 words </> 2 pts Question 13 p What were the absorbances of each of your three unknown replicates in the biuret protein assay? 1. 0.124 2. 0.116 3. 0.124 6 words </> 1

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 9/12 4 pts Question 14 Upload Your file has been successfully uploaded. Upload a picture of your BSA standard curve, with an appropriate line fitted to your data.  The standard curve 2.jpg 1 pts Question 15 p span Based on the average absorbance of your unknowns, and your standard curve, what is the concentration of protein in the sample you prepared, in mg/mL? A = εlc 0.1213 = 0.0459 x 1cm x c c = 0.1213 / 0.0459 = 2.6423 μM = 26.43 mg/mL 21 words </>

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3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 10/12 2 pts Question 16 p What is the concentration of protein in the original, undiluted calf serum, in mg/mL? C1V1 = C2V2 C1 x 1 = 26.43 X 10 C1 = 264.3 mg/mL 15 words </> 4 pts Question 17 Using the Henderson-Hasselbalch equation given below, and the setup details provided in the prac manual, calculate the theoretical pH of each tube, and enter them in the space provided rounded to 1 decimal place . The last one has been done for you. Tube pH

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 11/12 1 6.0 2 5.7 3 5.5 4 5.1 5 4.8 6 4.5 7 4.2 8 3.9 9 3.6 1 pts Question 18 Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6 Tube 7 Tube 8 Tube 9 Which tube showed the maximum turbidity/precipitation?

3/17/23, 2:54 AM Quiz: Prac 1 - Micropipetters and Spectrophotometry https://swinburne.instructure.com/courses/48474/quizzes/258673/take 12/12 Saved at 2:36 1 pts Question 19 Casein, pI = 4.7 Lactoglobulin, pI = 5.1 Pepsin, pI = 1.1 Chymotrypsin, pI = 8.3 Lysozyme, pI = 11 Based on your results, identify the protein from the list below, shown with their isoelectric points. Submit quiz

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Quiz_ Prac 1 - Micropipetters and Spectrophotometry

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