2024_ProblemSet_3 (1) (1)

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Apr 3, 2024

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1 Problem Set #3 Monday February 12 th , 2024 1. The R and S loci are 35 m.u. apart. If a plant with genotype ‘Rr Ss’ is selfed, what orogeny phenotypes will be seen, and in what proportions? 2. Chromosome 3 of corn carries three loci (b for plant-color booster, v for virescent, and lg for liguleless). A testcross of triple recessives with F 1 plants heterozygous for the three genes yields progeny having the following genotypes: 305 + v lg, 275 b + +, 128 b + lg, 112 + v +, 74 + + lg, 66 b v +, 22 + + +, and 18 b v lg. Give the gene sequence on the chromosome, the map distances between genes, and the coefficient of coincidence. 3. In corn a triple heterozygote was obtained carrying the mutant allele s (shrunken), w (white aleurone), and y (waxy endosperm), all paired with their normal wild-type alleles. This triple heterozygote was testcrossed, and the progeny contained; 116 shrunken white, 4 fully wild-type, 2538 shrunken, 601 shrunken waxy, 626 white, 2708 white waxy, 2 shrunken white waxy, and 113 waxy. a. Determine if any of these three loci are linked and, if so, show map distances. b. Show the allele arrangement on the chromosomes of the triple heterozygote used in the testcross. c. Calculate interference if appropriate. 4. A rice breeder obtained a triple heterozygote carrying three recessive alleles for albino flowers ( al ), brown awns ( b ), and fuzzy leaves ( fu ), all paired with their normal wild-type alleles. This triple heterozygote was testcrossed. The progeny phenotypes were: 170 wild-type 150 albino, brown, fuzzy 5 brown 3 albino, fuzzy 710 albino 698 brown, fuzzy 42 fuzzy 38 albino, brown d. Are any of the genes linked? If so, draw a map labelled with map distances. (Don’t bother with a correction for multiple crossovers). e. The triple heterozygote was originally made by crossing two pure lines. What were their genotypes? BIL250 Problem Set #3 Dr. Alex Wilson
2 5. A corn plant known to be hybrid for three linked genes is testcrossed. The progeny phenotypes are frequencies are: +++ 74 abc 70 a++ 44 +bc 50 ++c 4 ab+ 2 a+c 368 +b+ 388 1000 a. What is the gene order and gene arrangement of the trihybrid? (4 points) b. How many map units separate the loci? (Draw a picture) (2 points) c. What is the coefficient of coincidence for these loci? (2 points) 6. A newly identified fruit fly mutant, cyclops eye (one large eye in the middle of the head), is hypothesized to be autosomal dominant. The experimenter started with homozygous wild type females (yes, virgins) and homozygous cyclops males. The data from the F2 generation was 44 wild type males, 60 wild type females, 110 cyclops males and 150 cyclops females. Does this data support or reject the hypothesis? Use chi square to prove your position. BIL250 Problem Set #3 Dr. Alex Wilson
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