BIOL2000 Problem set Chapter 8

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Apr 3, 2024

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Q 31. Draw the longest continuous base-pairing interaction between the following RNAs: 5 -AAUGCCGGUAACGAUUAACGCCCGAUAUCCG-3 ′ ′ 5 -GAGCUUCCAUAUCGGGCGUUGGUGAUUCGAA-3 ′ ′ Q32. What role does the branch point ribose 2 -OH play in the splicing reaction? ′ Q 37. What is the primary function of the sigma factor in bacteria? Is there a factor in eukaryotes that is functionally analogous to the sigma factor? Q38. Write the sequence of the template and non-template strands of DNA that encode the following fragment of a bacterial mRNA: pppGUUCACUGGGACUAAAGCCCGGGAACUAGG Q39. Write the sequence of the template and non-template strands of DNA that encode the following eukaryotic mRNA, where the underlined sequence is the poly(A) tail: m7GpppGUUCACUGGGACUGAAUAAAGGGAACUAGGAAAAAAAAAAAAA(n = 150) Q 49. If you isolated an mRNA from a eukaryotic cell, what features would it have at its 5 and 3 ends if it ′ ′ is full length? Q50. If you had the sequence of an mRNA and the genome of a new organism, how would you determine the location in the genome of the transcription start site of the gene that encodes the mRNA? Q 53. In bacteria and eukaryotes, describe what else is happening to an mRNA while RNA polymerase is synthesizing it from the DNA template.
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